Characterizing N-acetylcysteine (NAC) and N-acetylcysteine amide (NACA) binding for lead poisoning treatment.
Characterizing N-acetylcysteine (NAC) and N-acetylcysteine amide (NACA) binding for lead poisoning treatment.
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DOI：
10.1016/j.jcis.2011.12.052
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发表时间：
2012-04-01
影响因子：
9.9
通讯作者：
Chusuei, Charles C.
中科院分区：
文献类型：
--
作者：
Chen, Weiqing;Ercal, Nuran;Tien Huynh;Volkov, Anatoliy;Chusuei, Charles C.
使用抗氧化剂是治疗铅中毒的重要手段。先前的体内研究表明，各种螯合剂抗氧化剂在降低血液铅（II）水平和铅中毒引起的氧化应激的能力之间存在显着差异。NAC和NACA的比较能力，铅（II）进行了研究，在体外，第一次，检查的作用-OH/-NH 2官能团的抗氧化剂结合行为。为了分析抗氧化剂-二价金属相互作用，将抗氧化剂探测为固体表面，将Pb（II）吸附到它们上。表面表征进行了使用X-射线光电子能谱（XPS）分析，以量化Pb（II）在所得的加合物。的Pb 4f轨道的XPS表明，更多的Pb（II）的化学键合到NACA比NAC。此外，通过NAC和NACA的零电荷点（PZC）测量来探测抗氧化剂表面，以进一步了解Pb-NAC和Pb-NACA结合，表明库仑相互作用起到了促进复合物形成的部分作用。数据与金属配体络合的溶液分析相关良好。紫外-可见光谱用于探测络合行为。发现NACA具有较高的结合亲和力，如从HPLC数据络合后溶液中可用的游离Pb（II）所示。电喷雾质谱（ESI-MS）用于描绘铅-抗氧化剂复合物的结构。实验结果进一步支持密度泛函理论（DFT）计算的超分子相互作用能（Einter）显示更大的相互作用的铅（II）与NACA比NAC。
Using antioxidants is an important means of treating lead poisoning. Prior in vivo studies showed marked differences between various chelator antioxidants in their ability to decrease both blood Pb(II) levels and oxidative stress resulting from lead poisoning. The comparative abilities of NAC and NACA to Pb(II) were studied in vitro, for the first time, to examine the role of the –OH/–NH2 functional group in antioxidant binding behavior. To assay the antioxidant-divalent metal interaction, the antioxidants were probed as solid surfaces, adsorbing Pb(II) onto them. Surface characterization was carried out using X-ray photoelectron spectroscopy (XPS) analysis to quantify Pb(II) in the resulting adducts. XPS of the Pb 4f orbitals showed that more Pb(II) was chemically bound to NACA than NAC. In addition, the antioxidant surfaces were probed via point-of-zero charge (PZC) measurements of NAC and NACA were obtained to gain further insight into the Pb-NAC and Pb-NACA binding, showing that Coulombic interactions played a partial role facilitating complex formation. The data correlated well with solution analysis of metal-ligand complexation. UV-vis spectroscopy was used to probe complexation behavior. NACA was found to have the higher binding affinity as shown by free Pb(II) available in solution after complexation from HPLC data. Electrospray ionization mass spectrometry (ESI-MS) was applied to delineate the structures of Pb-antioxidant complexes. Experimental results were further supported by density functional theory (DFT) calculations of supermolecular interaction energies (Einter) showing a greater interaction of Pb(II) with NACA than NAC.
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影响因子：
5.4
作者：
Odewumi CO;Badisa VL;Le UT;Latinwo LM;Ikediobi CO;Badisa RB;Darling-Reed SF
通讯作者：
Darling-Reed SF
影响因子：
4.4
作者：
HAY, PJ;WADT, WR
通讯作者：
WADT, WR
影响因子：
3.8
作者：
Sotelo, Norberto;de los Angeles Durazo, Maria;Dhanakotti, Nagasharmila
通讯作者：
Dhanakotti, Nagasharmila
影响因子：
3.5
作者：
Penugonda, Suman;Ercal, Nuran
通讯作者：
Ercal, Nuran
影响因子：
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作者：
Flora SJ
通讯作者：
Flora SJ