Generation of new salt-tolerant wheat lines and transcriptomic exploration of the responsive genes to ethylene and salt stress
Generation of new salt-tolerant wheat lines and transcriptomic exploration of the responsive genes to ethylene and salt stress
复制标题
耐盐小麦新品系的产生及乙烯和盐胁迫响应基因的转录组探索
DOI：
10.1007/s10725-021-00694-9
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发表时间：
2020-07
影响因子：
4.2
通讯作者：
Chun-Hai Dong
中科院分区：
文献类型：
--
作者：
Qian Ma;Huajian Zhou;Xinying Sui;Chunxue Su;Yanchong Yu;Hongbing Yang;Chun-Hai Dong
小麦（Triticum aestivumL.）是最重要的主食作物之一。大多数小麦品种对盐胁迫敏感，盐胁迫是限制小麦生产的主要因素。为了培育耐盐小麦品种，实现可持续粮食生产，我们使用甲基磺酸乙酯对小麦品种鲁源502的9万多粒种子进行了诱变。在盐碱地上筛选出2000个耐盐株系。我们进一步分析了所选植株的乙烯敏感性、盐相关生理变化和初步作物产量。我们发现了11个耐盐品系表现出乙烯不敏感性和高产量。转录组分析显示，3278个差异表达基因（DEG）在选定的突变体，包括编码CAB，PER/POD，BGLU，CYP 707和ZEP。大部分DEGs可能参与光合作用、次生代谢产物的合成、氰基氨基酸代谢、类胡萝卜素的合成、硫胺素代谢以及角质、枯草芽孢杆菌和蜡的生物合成途径。此外，在突变体中鉴定并分析了9个新的乙烯反应因子（ERFs）。这些ERFs可能在乙烯响应和耐盐性中起着关键作用。乙烯敏感性降低的突变体耐盐性增强，表明乙烯敏感性与耐盐性密切相关。
Wheat (Triticum aestivumL.) is one of the most important staple crops. Most of wheat varieties are sensitive to salt stress, which is a major limitation for wheat production. To develop salt-tolerant wheat varieties for sustainable grain production, we used ethylmethylsulfonate to mutagenize over 90,000 seeds of the wheat cultivar Luyuan502. A total of 2000 salt-tolerant lines were identified after screening the plants in a salinized field. We further analyzed ethylene sensitivity, salt related physiological changes, and preliminary crop yield of the selected plants. We found 11 salt-tolerant lines exhibiting ethylene insensitivity and high grain production. Transcriptome analysis revealed 3278 differently expressed genes (DEGs) in the selected mutants, including the ones encoding CABs, PERs/PODs, BGLUs, CYP707s, and ZEPs. Most of DEGs may be involved in photosynthesis, biosynthesis of secondary metabolites, cyanoamino acid metabolism, carotenoid biosynthesis, thiamine metabolism, and cutin, suberine and wax biosynthesis pathways. In addition, 9 novelETHYLENE RESPONSE FACTORs(ERFs) were identified and analyzed in the mutants. These ERFs may play critical roles in ethylene response and salt tolerance. The mutant lines with decreased ethylene sensitivity exhibited enhanced salt tolerance, suggesting that ethylene sensitivity was closely related with salt tolerance.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.2
作者：
Youning Wang;Tao Wang;Kexue Li;Xia Li
通讯作者：
Youning Wang;Tao Wang;Kexue Li;Xia Li
影响因子：
5.3
作者：
Dongmiao Liu;Caixia Han;Xiong Deng;Yue Liu;Nannan Liu;Yueming Yan
通讯作者：
Dongmiao Liu;Caixia Han;Xiong Deng;Yue Liu;Nannan Liu;Yueming Yan
影响因子：
56.9
作者：
BLEECKER, AB;ESTELLE, MA;KENDE, H
通讯作者：
KENDE, H