Early exposure to sevoflurane inhibits Ca2+ channels activity in hippocampal CA1 pyramidal neurons of developing rats
Early exposure to sevoflurane inhibits Ca2+ channels activity in hippocampal CA1 pyramidal neurons of developing rats
复制标题
早期接触七氟烷会抑制发育中大鼠海马 CA1 锥体神经元的 Ca2 通道活性
DOI：
10.1016/j.brainres.2014.02.008
复制
发表时间：
2014-04
期刊：
影响因子：
2.9
通讯作者：
Tian Xin
中科院分区：
文献类型：
--
作者：
Liu Aili;Li Yize;Tan Tao;Tian Xin
Sevoflurane is one of inhalation anesthetics and has been commonly used in obstetric and pediatric anesthesia. The widespread use of sevoflurane in newborns and infants has made its safety a health issue of concern. Voltage-gated Ca2+channels (VGCCs) play an important role in neuronal excitability and are essential for normal brain development. However, the role of sevoflurane on regulating Ca2+channels during the period of rapid brain development is still not well understood.The aim of this study is to explore the effects of sevoflurane on voltage-gated Ca2+channels for hippocampal CA1 pyramidal neurons during the period of rapid brain development. 1-week-old Sprague-Dawley rats were randomly divided into 3 groups: control group, 2.1% sevoflurane group (exposed to 2.1% sevoflurane for 6 h) and 3% sevoflurane group (exposed to 3% sevoflurane for 6 h). Whole-cell patch clamp technique was used.I–Vcurve, steady-state activation and inactivation curves of Ca2+channels were studied in rats of the both 3 treated groups at 5 different ages (1 week, 2 weeks, 3 weeks, 4 and 5 weeks old).After anesthesia with sevoflurane at 1-week-old rats, Ca2+channels current density was significantly decreased at week 1 and week 2 (p<0.01). And 3% sevoflurane exposure resulted in a rightward shift in steady-state activation curve at week 1 and week 2, as well as the inactivation curve from week 1 to week 3. However, the 2.1% sevoflurane-induced rightward shift was only found in steady-state inactivation curve of Ca2+channels at week 1 and week 2. Both the slope factor (k) of Ca2+channels activation and inactivation curves increased by 3% sevoflurane at week 1 (p<0.05).Therefore, early exposure to sevoflurane persistently inhibits Ca2+channels activity in hippocampal CA1 pyramidal neurons of developing rats but the development of Ca2+channels recovers to normal level at juvenile age. Moreover, the inhibition of 3% sevoflurane on VGCCs is greater than that of 2.1% sevoflurane.
登录
查看更多内容
影响因子：
4.7
作者：
Eckle VS;Digruccio MR;Uebele VN;Renger JJ;Todorovic SM
通讯作者：
Todorovic SM
影响因子：
2.2
作者：
Brosnan RJ;Thiesen R
通讯作者：
Thiesen R
影响因子：
3.3
作者：
Yu-Hsueh Wang;Yongxia Cheng;Guibo Liu;Xiaodan Tian;Xuefeng Tu;Junke Wang
通讯作者：
Yu-Hsueh Wang;Yongxia Cheng;Guibo Liu;Xiaodan Tian;Xuefeng Tu;Junke Wang
影响因子：
3.3
作者：
M. Taketo;T. Yoshioka
通讯作者：
M. Taketo;T. Yoshioka
影响因子：
8.8
作者：
Shen X;Dong Y;Xu Z;Wang H;Miao C;Soriano SG;Sun D;Baxter MG;Zhang Y;Xie Z
通讯作者：
Xie Z