Colonic inflammation up-regulates voltage-gated sodium channels in bladder sensory neurons via activation of peripheral transient potential vanilloid 1 receptors.
Colonic inflammation up-regulates voltage-gated sodium channels in bladder sensory neurons via activation of peripheral transient potential vanilloid 1 receptors.
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结肠炎症上调通过激活外周瞬态潜在的香草素1受体的膀胱感觉神经元中的电压门控通道。
DOI：
10.1111/j.1365-2982.2012.01910.x
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发表时间：
2012-06
影响因子：
3.5
通讯作者：
Malykhina AP
中科院分区：
文献类型：
--
作者：
Lei Q;Malykhina AP
Primary sensory neurons express several types of ion channels including transient receptor potential vanilloid 1 (TRPV1) and voltage-gated Na+ channels. Our previous studies showed an increased excitability of bladder primary sensory and spinal neurons triggered by inflammation in the distal colon as a result of pelvic organ cross-sensitization. The goal of this work was to determine the effects of TRPV1 receptor activation by potent agonists and/or colonic inflammation on voltage-gated Na+ channels expressed in bladder sensory neurons. Sprague-Dawley rats were treated with intracolonic saline (control), resiniferatoxin (RTX, 10−7 M), TNBS (colonic irritant) or double treatment (RTX followed by TNBS). TNBS-induced colitis increased the amplitude of total Na+ current by 2-fold and of tetrodotoxin resistant (TTX-R) Na+ current by 78% (p≤0.05 to control) in lumbosacral bladder neurons during acute phase (3 days post-TNBS). Instillation of RTX in the distal colon caused an enhancement in the amplitude of total Na+ current at −20 mV from −112.1±18.7 pA/pF (control) to −183.6±27.8 pA/pF (3 days post-RTX, p≤0.05) without changes in TTX resistant component. The amplitude of net Na+ current was also increased by 119% at day 3 in the group with double treatment (RTX followed by TNBS, p≤0.05 to control) which was significantly higher than in either group with a single treatment. These results provide evidence that colonic inflammation activates TRPV1 receptors at the peripheral sensory terminals leading to an up-regulation of voltage gated Na+ channels on the cell soma of bladder sensory neurons. This mechanism may underlie the occurrence of peripheral cross-sensitization in the pelvis and functional chronic pelvic pain.
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影响因子：
4
作者：
Devor, M
通讯作者：
Devor, M
影响因子：
5.3
作者：
Amir, R;Michaelis, M;Devor, M
通讯作者：
Devor, M
影响因子：
5.3
作者：
Amir, R;Kocsis, JD;Devor, M
通讯作者：
Devor, M
影响因子：
2.7
作者：
Brumovsky, P. R.;Gebhart, G. F.
通讯作者：
Gebhart, G. F.
DOI：
10.1152/ajpregu.00804.2010
发表时间：
2011-06-01
影响因子：
2.8
作者：
Asfaw, Tirsit S.;Hypolite, Joseph;Malykhina, Anna P.
通讯作者：
Malykhina, Anna P.