Selective regulation of human TRAAK channels by biologically active phospholipids.
Selective regulation of human TRAAK channels by biologically active phospholipids.
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通过生物活性磷脂对人Traak通道的选择性调节。
DOI：
10.1038/s41589-020-00659-5
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发表时间：
2021-01
影响因子：
14.8
通讯作者：
Laganowsky A
中科院分区：
文献类型：
--
作者：
Schrecke S;Zhu Y;McCabe JW;Bartz M;Packianathan C;Zhao M;Zhou M;Russell D;Laganowsky A
TRAAK is an ion channel from the two-pore domain potassium (K2P) channel family with roles in maintaining the resting membrane potential and fast action potential conduction. Regulated by a wide range of physical and chemical stimuli, the affinity and selectivity of K2P4.1 towards lipids remains poorly understood. Here we show the two isoforms of K2P4.1 have distinct binding preferences for lipids dependent on acyl chain length and position on the glycerol backbone. Unexpectedly, the channel can also discriminate the fatty acid linkage at the sn-1 position. Of the 33 lipids interrogated using native mass spectrometry, phosphatidic acid (PA) had the lowest equilibrium dissociation constants for both isoforms of K2P4.1. Liposome potassium flux assays with K2P4.1 reconstituted in defined lipid environments show that those containing PA activate the channel in a dose-dependent fashion. Our results begin to define the molecular requirements for the specific binding of lipids to K2P4.1.
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影响因子：
16.6
作者：
Cong X;Liu Y;Liu W;Liang X;Laganowsky A
通讯作者：
Laganowsky A
DOI：
10.1073/pnas.1407160111
发表时间：
2014-09-16
影响因子：
11.1
作者：
Comoglio, Yannick;Levitz, Joshua;Sandoz, Guillaume
通讯作者：
Sandoz, Guillaume
影响因子：
15
作者：
Cong, Xiao;Liu, Yang;Laganowsky, Arthur
通讯作者：
Laganowsky, Arthur
影响因子：
16.6
作者：
Allison TM;Reading E;Liko I;Baldwin AJ;Laganowsky A;Robinson CV
通讯作者：
Robinson CV
影响因子：
20.1
作者：
Blondeau, Nicolas;Petrault, Olivier;Heurteaux, Catherine
通讯作者：
Heurteaux, Catherine