Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous system.
Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous system.
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DOI：
10.7554/elife.79156
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发表时间：
2023-10-25
期刊：
影响因子：
7.7
通讯作者：
Bronner ME
中科院分区：
文献类型：
--
作者：
Jacobs-Li J;Tang W;Li C;Bronner ME
在发育过程中，肠神经系统（ENS）的大部分来自迷走神经嵴，迷走神经嵴从尾部后脑出现，并在整个胃肠道定植。然而，第二个ENS贡献来自骶神经嵴，骶神经嵴出现在尾神经管中并填充后脐肠。通过偶联单细胞转录组学与轴向水平特异性谱系跟踪在禽胚胎，我们比较了胚胎迷走神经和骶神经嵴细胞的鸡ENS和相关的外周神经节（雷马克神经和骨盆丛）的贡献。在胚胎第10天，两个神经嵴群体形成神经元和神经胶质细胞类型的重叠子集。令人惊讶的是，脐后迷走神经嵴比脐前迷走神经嵴更接近骶神经嵴。然而，迷走神经和骶源性细胞之间的集群类型的一些差异。值得注意的是，RNA轨迹分析表明，迷走神经嵴保持神经元/神经胶质祖细胞池，而这一簇在E10骶神经嵴中被耗尽，而E10骶神经嵴则具有许多肠神经胶质。本研究结果揭示了骶神经嵴的贡献后肠和相关的外周神经节，并强调了潜在的影响，当地环境和/或发展的时间在神经嵴衍生细胞的分化在发展中的ENS。
During development, much of the enteric nervous system (ENS) arises from the vagal neural crest that emerges from the caudal hindbrain and colonizes the entire gastrointestinal tract. However, a second ENS contribution comes from the sacral neural crest that arises in the caudal neural tube and populates the post-umbilical gut. By coupling single-cell transcriptomics with axial-level-specific lineage tracing in avian embryos, we compared the contributions of embryonic vagal and sacral neural crest cells to the chick ENS and the associated peripheral ganglia (Nerve of Remak and pelvic plexuses). At embryonic day (E) 10, the two neural crest populations form overlapping subsets of neuronal and glia cell types. Surprisingly, the post-umbilical vagal neural crest much more closely resembles the sacral neural crest than the pre-umbilical vagal neural crest. However, some differences in cluster types were noted between vagal and sacral derived cells. Notably, RNA trajectory analysis suggests that the vagal neural crest maintains a neuronal/glial progenitor pool, whereas this cluster is depleted in the E10 sacral neural crest which instead has numerous enteric glia. The present findings reveal sacral neural crest contributions to the hindgut and associated peripheral ganglia and highlight the potential influence of the local environment and/or developmental timing in differentiation of neural crest-derived cells in the developing ENS.
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影响因子：
5.6
作者：
Ritter KE;Buehler DP;Asher SB;Deal KK;Zhao S;Guo Y;Southard-Smith EM
通讯作者：
Southard-Smith EM
影响因子：
3.1
作者：
Das, Jayanta K.;Voelkel, Norbert F.;Felty, Quentin
通讯作者：
Felty, Quentin
PAX3脊柱裂裂突变体中的s骨神经rest发育改变了膀胱神经和功能的缺陷。
影响因子：
2.7
作者：
Deal KK;Chandrashekar AS;Beaman MM;Branch MC;Buehler DP;Conway SJ;Southard-Smith EM
通讯作者：
Southard-Smith EM
影响因子：
5.6
作者：
Ji Y;Tam PK;Tang CS
通讯作者：
Tang CS