Rapid odor perception in rat olfactory bulb by microelectrode array
Rapid odor perception in rat olfactory bulb by microelectrode array
复制标题
微电极阵列快速感知大鼠嗅球的气味
DOI：
10.1631/jzus.b1200073
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Wang Ping
中科院分区：
文献类型：
--
作者：
Zhou Jun;Dong Qi;Zhuang Liujing;Li Rong;Wang Ping
用16通道微电极阵列记录了42只麻醉自由呼吸大鼠嗅球内302个M/T细胞的反应。应用四种纯化学品的饱和蒸气，苯甲醚、香芹酮、柠檬醛和乙酸异戊酯。在气味发作后的吸入的初始阶段对齐的尖峰列车后，M/T细胞的反应表现出短暂的时间特征，包括兴奋和抑制模式。这两种气味诱发的模式表明，哺乳动物识别气味刺激后的短呼吸周期内。由于从单个细胞接收到的信息量很小，我们汇集了所有响应性M/T细胞的结果以研究整体活性。在100 ms区间内计算细胞系综的放电率，并构建群体向量。使用主成分分析将高维向量压缩成三维以用于可视化。在气味刺激过程中，兴奋性和抑制性细胞系综的轨迹显示出强烈的动力学。聚类中心之间的距离比静息状态下的距离增大。因此，我们推测，气味信息的图片发送到更高的大脑区域被描绘和气味辨别是在第一个呼吸周期内完成。
Responses of 302 mitral/tufted (M/T) cells in the olfactory bulb were recorded from 42 anesthetized freely breathing rats using a 16-channel microwire electrode array. Saturated vapors of four pure chemicals, anisole, carvone, citral and isoamyl acetate were applied. After aligning spike trains to the initial phase of the inhalation after odor onset, the responses of M/T cells showed transient temporal features including excitatory and inhibitory patterns. Both odor-evoked patterns indicated that mammals recognize odors within a short respiration cycle after odor stimulus. Due to the small amount of information received from a single cell, we pooled results from all responsive M/T cells to study the ensemble activity. The firing rates of the cell ensembles were computed over 100 ms bins and population vectors were constructed. The high dimension vectors were condensed into three dimensions for visualization using principal component analysis. The trajectories of both excitatory and inhibitory cell ensembles displayed strong dynamics during odor stimulation. The distances among cluster centers were enlarged compared to those of the resting state. Thus, we presumed that pictures of odor information sent to higher brain regions were depicted and odor discrimination was completed within the first breathing cycle.
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影响因子：
9.2
作者：
M. Stopfer
通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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