Cell cycles and proliferation patterns in Haematococcus pluvialis

Cell cycles and proliferation patterns in Haematococcus pluvialis
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雨生红球藻的细胞周期和增殖模式

DOI:
10.1007/s00343-017-6103-8
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发表时间:
2017-09
影响因子:
--
通讯作者:
Litao Zhang
Litao Zhang
中科院分区:
地学4区
文献类型:
--
作者:
Chunhui Zhang;Jianguo Liu;Litao Zhang

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对雨生红球藻的研究大多集中在细胞生长和虾青素的积累上,对细胞周期和增殖模式的研究较少。这项研究的目的是利用摄像和录像系统在显微镜下阐明雨生隐翅虫的细胞周期和增殖模式。雨生隐翅虫复杂的生活史可分为两个阶段:活动阶段和非活动阶段。所有的细胞都可分为活动细胞、非活动细胞、游动孢子和无轮孢子。无论是在运动期还是非运动期,雨生螺旋藻的主要细胞增殖都是通过无性繁殖实现的。在正常生长条件下,一个活动细胞通常会产生两个,有时是四个,甚至是八个游动孢子。在不利的条件下,运动细胞失去鞭毛,转变为非运动细胞,非运动细胞通常产生2、4或8个无性孢子,偶尔产生20-32个无性孢子,进一步发育为非运动性细胞。在适当的条件下,非运动细胞也能够释放游动孢子。较大的非游动细胞产生16个以上游动孢子,较小的细胞产生4到8个游动孢子。营养生殖是在运动阶段通过直接细胞分裂,在非运动阶段通过偶尔的细胞萌发。到目前为止,还没有令人信服的直接证据证明有性繁殖。
Most studies on Haematococcus pluvialis have been focused on cell growth and astaxanthin accumulation; far less attention has been paid to cell cycles and proliferation patterns. The purpose of this study was to clarify cell cycles and proliferation patterns in H. pluvialis microscopically using a camera and video recorder system. The complicated life history of H. pluvialis can be divided into two stages: the motile stage and the non-motile stage. All the cells can be classified into forms as follows: motile cell, nonmotile cell, zoospore and aplanospore. The main cell proliferation, both in the motile phase and non-motile phase in H. pluvialis, is by asexual reproduction. Under normal growth conditions, a motile cell usually produces two, sometimes four, and exceptionally eight zoospores. Under unfavorable conditions, the motile cell loses its flagella and transforms into a non-motile cell, and the non-motile cell usually produces 2, 4 or 8 aplanospores, and occasionally 20–32 aplanospores, which further develop into non-motile cells. Under suitable conditions, the non-motile cell is also able to release zoospores. The larger non-motile cells produce more than 16 zoospores, and the smaller ones produce 4 or 8 zoospores. Vegetative reproduction is by direct cell division in the motile phase and by occasional cell budding in the non-motile phase. There is, as yet, no convincing direct evidence for sexual reproduction.
DOI: 10.1007/s12010-009-8627-7
发表时间: 2010-03
影响因子: 3
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