Low-intensity pulsed ultrasound induces angiogenesis and ameliorates left ventricular dysfunction in a porcine model of chronic myocardial ischemia.
Low-intensity pulsed ultrasound induces angiogenesis and ameliorates left ventricular dysfunction in a porcine model of chronic myocardial ischemia.
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DOI：
10.1371/journal.pone.0104863
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Shimokawa H
中科院分区：
文献类型：
--
作者：
Hanawa K;Ito K;Aizawa K;Shindo T;Nishimiya K;Hasebe Y;Tuburaya R;Hasegawa H;Yasuda S;Kanai H;Shimokawa H
尽管缺血性心脏病（IHD）的治疗取得了显著进展，但严重IHD患者的数量仍在增加。因此，开发新的非侵入性治疗策略至关重要。在本研究中，我们的目的是开发低强度脉冲超声（LIPUS）治疗IHD。我们首次证实LIPUS可显著上调培养的人内皮细胞血管内皮生长因子（VEGF）mRNA的表达，在32周期时达到峰值（P<0.05）。然后，我们研究了LIPUS在慢性心肌缺血伴左心室射血分数（LVEF）降低的猪模型（n = 28）中的体内效应。 在3个不同的短轴水平，用假手术（n = 14）或LIPUS（32个周期，193 mW/cm 2，20 min，n =14）处理心脏。 治疗4周后，LIPUS组左室射血分数（LVEF）明显改善（46±4 ~ 57± 5%，P<0.05），而假手术组左室射血分数（LVEF）无明显变化（46±5 ~ 47± 6%，P = 0.33）。 LIPUS组缺血区毛细血管密度（1084±175）个/mm 2，明显高于对照组（858±151）个/mm 2（P<0.05）。局部心肌血流量在LIPUS组也有明显改善（0.78±0.2 ~ 1.39± 0.4ml/min/g，P<0.05），而对照组无明显改善（0.84±0.3 ~ 0.97± 0.4ml/min/g）。Western blot结果显示，VEGF、eNOS和bFGF仅在LIPUS组表达显著上调。这些结果表明，LIPUS治疗是一种新的，非侵入性治疗IHD有前途。
Although a significant progress has been made in the management of ischemic heart disease (IHD), the number of severe IHD patients is increasing. Thus, it is crucial to develop new, non-invasive therapeutic strategies. In the present study, we aimed to develop low-intensity pulsed ultrasound (LIPUS) therapy for the treatment of IHD. We first confirmed that in cultured human endothelial cells, LIPUS significantly up-regulated mRNA expression of vascular endothelial growth factor (VEGF) with a peak at 32-cycle (P<0.05). Then, we examined the in vivo effects of LIPUS in a porcine model of chronic myocardial ischemia with reduced left ventricular ejection fraction (LVEF) (n = 28). The heart was treated with either sham (n = 14) or LIPUS (32-cycle with 193 mW/cm2 for 20 min, n = 14) at 3 different short axis levels. Four weeks after the treatment, LVEF was significantly improved in the LIPUS group (46±4 to 57±5%, P<0.05) without any adverse effects, whereas it remained unchanged in the sham group (46±5 to 47±6%, P = 0.33). Capillary density in the ischemic region was significantly increased in the LIPUS group compared with the control group (1084±175 vs. 858±151/mm2, P<0.05). Regional myocardial blood flow was also significantly improved in the LIPUS group (0.78±0.2 to 1.39±0.4 ml/min/g, P<0.05), but not in the control group (0.84±0.3 to 0.97±0.4 ml/min/g). Western blot analysis showed that VEGF, eNOS and bFGF were all significantly up-regulated only in the LIPUS group. These results suggest that the LIPUS therapy is promising as a new, non-invasive therapy for IHD.
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影响因子：
3.3
作者：
Serizawa, Fukashi;Ito, Kenta;Satomi, Susumu
通讯作者：
Satomi, Susumu
影响因子：
1.8
作者：
Uwatoku, Toyokazu;Ito, Kenta;Shimokawa, Hiroaki
通讯作者：
Shimokawa, Hiroaki
影响因子：
1.8
作者：
Fukumoto, Y;Ito, A;Shimokawa, H
通讯作者：
Shimokawa, H
影响因子：
3.7
作者：
Fu M;Sun CK;Lin YC;Wang CJ;Wu CJ;Ko SF;Chua S;Sheu JJ;Chiang CH;Shao PL;Leu S;Yip HK
通讯作者：
Yip HK
DOI：
10.1016/j.ejvs.2011.02.029
发表时间：
2011-08-01
影响因子：
5.7
作者：
Serizawa, F.;Ito, K.;Satomi, S.
通讯作者：
Satomi, S.