Identification and characterization of expressed TIR- and non-TIR-NBS-LRR resistance gene analogous sequences from radish (Raphanus sativus L.) de novo transcriptome
Identification and characterization of expressed TIR- and non-TIR-NBS-LRR resistance gene analogous sequences from radish (Raphanus sativus L.) de novo transcriptome
复制标题
萝卜 (Raphanus sativus L.) 转录组中表达的 TIR-和非 TIR-NBS-LRR 抗性基因类似序列的鉴定和表征
DOI：
10.1016/j.scienta.2016.12.019
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发表时间：
2017-02
影响因子：
4.3
通讯作者：
Liu Liwang
中科院分区：
文献类型：
--
作者：
Wang Yan;Song Zhaohui;Zhang Wei;Xu Liang;Su Xiaojun;Muleke Everlyne M'mbone;Liu Liwang
萝卜是世界范围内重要的根茎类蔬菜作物。然而，它容易受到各种疾病的影响，导致产量和品质下降。植物核苷酸结合位点(NBS)-富亮氨酸重复序列(LRR)基因超家族对多种病原菌有效。因此，分离和鉴定这类基因类似物(RGA)是提高植物抗病能力的重要基础。然而，到目前为止，还没有在萝卜中全面分离NBS-LRR RGA的报道。在本研究中，从萝卜叶片转录组中获得了36,613个单基因，代表81,481个独特的转录本，平均长度为1253.22个核苷酸。通过生物信息学分析，共鉴定出38个NBS编码序列。多序列比对分析将这些序列分为Toll和IL-1受体(TIR)-NBS-LRR和非TIR-NBS-LRR亚类。对推导出的氨基酸序列进行系统发育树分析，将这些序列分为六类。与同义替换率的不同表明，萝卜中RGA的NBS编码序列受到纯化或负选择的影响。此外，RT-qPCR分析表明，几个RsRGA在三种非生物刺激(SA、MeJA和ABA)下存在差异表达，这表明它们可能通过激活信号通路参与防御反应。这些结果可为进一步鉴定植物RGA提供有价值的信息，加速萝卜抗病育种的遗传改良。
Radish is an important root vegetable crop worldwide. However, it is susceptible to a variety of diseases leading to decline in yield and quality. The plant nucleotide-binding site (NBS)-leucine-rich repeat (LRR) gene superfamily is effective against a big range of pathogens. Therefore, the isolation and identification of suchRgene analogue (RGA) is a critical foundation for improving disease resistance in plants. So far, however, no comprehensive isolation of NBS-LRR RGAs has been reported in radish. In this study, a total of 36,613 unigenes representing 81,481 unique transcripts with an average length of 1253.22 nt were obtained from radish leaf transcriptome. In total, 38 NBS-encoding sequences were successfully identified via bioinformatics analysis. Multiple sequence alignment analysis classified these sequences into toll and interleukin-1 receptor (TIR)-NBS-LRR and non-TIR-NBS-LRR subclasses based on the presence of a TIR domain at the N-terminus. Phylogenetic tree analysis of the deduced amino acid sequences grouped these sequences into six classes. Non-synonymous to synonymous substitution ratio suggested that NBS-encoding sequences of RGAs in radish were subject to purification or negative selection. Additionally, RT-qPCR analysis revealed that severalRsRGAsexpressed differentially under three tested abiotic stimuli (SA, MeJA and ABA), suggesting that they might be involved in defense responses by activating signaling pathways. These results could provide valuable information for further identification of plant RGAs and accelerate genetic improvement of disease resistances in radish breeding programs.
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黄瓜中潜在 NBS 编码抗性基因的鉴定和表征以及与霜霉病抗性相关的假定候选基因的诱导动力学
影响因子：
5.3
作者：
Wan H;Zhao Z;Malik AA;Qian C;Chen J
通讯作者：
Chen J
DOI：
10.1007/bf02879332
发表时间：
2001-06
期刊：
Science in China Series C: Life Sciences
影响因子：
--
作者：
Xianwu Zheng;W. Zhai;Xiaobing Li;Wenjun Wang;Jichen Xu;Guozhen Liu;Lihuang Zhu
通讯作者：
Xianwu Zheng;W. Zhai;Xiaobing Li;Wenjun Wang;Jichen Xu;Guozhen Liu;Lihuang Zhu
影响因子：
5.3
作者：
Losada JM;Herrero M
通讯作者：
Herrero M
影响因子：
5.4
作者：
Liang Xu;Liang-ju Wang;Y. Gong;W. Dai;Yan Wang;Xianwen Zhu;Tiancai Wen;Liwang Liu
通讯作者：
Liang Xu;Liang-ju Wang;Y. Gong;W. Dai;Yan Wang;Xianwen Zhu;Tiancai Wen;Liwang Liu
影响因子：
3.9
作者：
Cannon, SB;Zhu, HY;Young, ND
通讯作者：
Young, ND