Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth.
Glycoprotein composition along the pistil of Malus x domestica and the modulation of pollen tube growth.
复制标题
DOI：
10.1186/1471-2229-14-1
复制
发表时间：
2014-01-03
影响因子：
5.3
通讯作者：
Herrero M
中科院分区：
文献类型：
--
作者：
Losada JM;Herrero M
花粉管的生长特征不是恒定的，在雌蕊的不同组织中表现出不同的生长模式。柱头生长缓慢，自养，花柱生长迅速，异养。这些不同的母体组织与穿越花粉管之间的相互作用以及这种相互作用对所观察到的代谢的作用知之甚少。在这项工作中，我们表征了苹果花的花粉管生长，并寻找两种不同母体组织，柱头和花柱之间糖蛋白表位定位的差异。虽然免疫细胞化学检测到的阿拉伯半乳聚糖蛋白在柱头中含量很高，但在柱头的传递组织中却没有检测到，因为柱头的延伸蛋白丰富。而伸展蛋白在未授粉的雌蕊中保持高水平，在花柱通过花粉管后不再存在。同样，在未授粉花柱中，β-葡聚糖等不溶性多糖在授粉雌蕊中含量丰富。花粉管生长从自养型向异养型的转变在空间上与柱头和花柱之间糖蛋白表位的变化有关。花柱中花粉管传代后伸展蛋白和多糖的消耗可能对异养花粉管生长的加速有贡献，这可能意味着雌性组织对前合子雌雄串扰有积极的贡献。
The characteristics of pollen tube growth are not constant, but display distinct patterns of growth within the different tissues of the pistil. In the stigma, the growth rate is slow and autotrophic, whereas in the style, it is rapid and heterotrophic. Very little is known about the interactions between these distinct maternal tissues and the traversing pollen tube and the role of this interaction on the observed metabolism. In this work we characterise pollen tube growth in the apple flower and look for differences in glycoprotein epitope localization between two different maternal tissues, the stigma and the style. While immunocytochemically-detected arabinogalactan proteins were present at high levels in the stigma, they were not detected in the transmitting tissue of the style, where extensins were abundant. Whereas extensins remained at high levels in unpollinated pistils, they were no longer present in the style following pollen tube passage. Similarily, while abundant in unpollinated styles, insoluble polysaccharides such as β-glucans, were depleted in pollinated pistils. The switch from autotropic to heterotrophic pollen tube growth correlates spatially with a change of glycoprotein epitopes between the stigma and the style. The depletion of extensins and polysaccharides following pollen tube passage in the style suggest a possible contribution to the acceleration of heterotrophic pollen tube growth, which would imply an active contribution of female tissues on prezygotic male–female crosstalk.
登录
查看更多内容
DOI：
10.1073/pnas.0711980105
发表时间：
2008-02-12
影响因子：
11.1
作者：
Cannon, Maura C.;Terneus, Kimberly;Kieliszewski, Marcia J.
通讯作者：
Kieliszewski, Marcia J.
影响因子：
4.2
作者：
ARBELOA, A;HERRERO, M
通讯作者：
HERRERO, M
影响因子：
7.4
作者：
Dardelle, Flavien;Lehner, Arnaud;Mollet, Jean-Claude
通讯作者：
Mollet, Jean-Claude
影响因子：
--
作者：
de Graaf, BHJ;Knuiman, BA;Mariani, C
通讯作者：
Mariani, C
影响因子：
6.9
作者：
Chae K;Gonong BJ;Kim SC;Kieslich CA;Morikis D;Balasubramanian S;Lord EM
通讯作者：
Lord EM