Changes in rat myometrial plasma membrane protein kinase A are confined to parturition.
Changes in rat myometrial plasma membrane protein kinase A are confined to parturition.
复制标题
DOI：
10.1177/1933719110368869
复制
发表时间：
2010-07
期刊：
影响因子：
--
通讯作者：
Sanborn BM
中科院分区：
文献类型：
--
作者：
Ku CY;Murtazina DA;Kim YS;Garfield RE;Sanborn BM
我们以前已经表明，妊娠大鼠子宫肌层质膜相关的cAMP依赖性蛋白激酶A（PKA）减少交付前，与cAMP对收缩刺激参数的抑制作用下降一致。我们现在发现，大鼠子宫肌膜相关PKA浓度在妊娠早期至中期相当于骑自行车的大鼠。随着分娩相关的下降，膜PKA在产后1-2天内恢复。与未处理的对照组相比，用抗孕激素奥那司酮治疗12 h后，子宫肌层膜PKA催化和调节亚基减少。这与最近报道的电活动和收缩活动的增加在时间上一致。在单侧妊娠大鼠，质膜PKA的下降，观察到在非妊娠和妊娠角，但更迅速的妊娠角。这些数据表明，子宫肌层质膜PKA模式之前和在大多数怀孕是不一致的孕激素发挥主要影响PKA膜定位。相反，与分娩相关的膜PKA的下降可能有助于或受分娩的收缩和电活动增加的影响，这是孕酮影响丧失的结果，并不完全依赖于胎儿的存在。
We have previously shown that pregnant rat myometrial plasma membrane-associated cAMP-dependent protein kinase A (PKA) decreases prior to delivery, coincident with a decline in the inhibitory effect of cAMP on contractant-stimulated parameters. We now find that rat myometrial membrane-associated PKA concentrations in early to mid-pregnancy are equivalent to those in cycling rats. Following the decline associated with parturition, membrane PKA recovers within 1–2 days postpartum. Treatment with the antiprogestin onapristone caused a decrease in myometrial membrane PKA-catalytic and regulatory subunits compared to untreated controls by 12 h. This coincided temporally with recently reported increases in electrical and contractile activity. In unilaterally pregnant rats, the decline in plasma membrane PKA was observed in both nonpregnant and pregnant horns but was more rapid in the pregnant horns. These data indicate that the myometrial plasma membrane PKA pattern before and during most of pregnancy is not consistent with progesterone exerting a primary influence on PKA membrane localization. Rather, the fall in membrane PKA associated with parturition may contribute to or be influenced by the increased contractile and electrical activity of labor that is a consequence of the loss of progesterone influence and is not absolutely dependent on the presence of fetuses.
登录
查看更多内容
影响因子：
6.2
作者：
Challis, JRG
通讯作者：
Challis, JRG
影响因子：
5.5
作者：
Arthur, Patrice;Taggart, Michael J.;Mitchell, Bryan F.
通讯作者：
Mitchell, Bryan F.
影响因子：
4.8
作者：
Manni, Sabrina;Mauban, Joseph H.;Bond, Meredith
通讯作者：
Bond, Meredith
影响因子：
9.8
作者：
Shi, Shao-Qing;Maner, William L.;Garfield, Robert E.
通讯作者：
Garfield, Robert E.
影响因子：
4.8
作者：
KU, CY;QIAN, A;SANBORN, BM
通讯作者：
SANBORN, BM