Identification and distribution of immunocompetent cells in inflamed gingiva of human chronic periodontitis
Identification and distribution of immunocompetent cells in inflamed gingiva of human chronic periodontitis
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人慢性牙周炎发炎牙龈中免疫活性细胞的鉴定和分布
DOI：
10.1128/iai.41.1.365-374.1983
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发表时间：
1983
影响因子：
3.1
通讯作者：
H. Yamagami
中科院分区：
文献类型：
--
作者：
H. Okada;T. Kida;H. Yamagami
晚期人类牙周炎被认为是B细胞病变，但细胞浸润包含几种细胞类型，其分布尚未确定。本实验旨在表征和鉴定组织学切片上和来自患病人牙龈的洗脱液中的免疫活性细胞。用对人免疫球蛋白G（IgG）、伊加或IgM具有单特异性的F（ab '）2抗血清通过直接免疫荧光在组织学切片上检测免疫球蛋白携带细胞。浆细胞在固有层的中心部分占优势，IgG、伊加和IgM阳性的比例分别占总浸润细胞的65.2 +/-9.5%、11.2 +/-1.1%和1.3 +/- 1.1%。通过抗人T细胞单克隆抗体（Leu-1）的间接免疫荧光鉴定的T淋巴细胞占总浸润细胞的29.3 +/- 10.0%。大部分T细胞位于囊袋上皮下，但也有少数在中央固有层。同样，Fc受体轴承细胞检测EA玫瑰花结和巨噬细胞和单核细胞检测非特异性酯酶染色与α-萘丁酸酯酶也定位于该地区紧邻口袋上皮。用胶原酶、透明质酸酶和DNA酶消化后，从切碎的牙龈组织中收获浸润细胞。洗脱液含有35.3 +/- 6.0% T淋巴细胞、30.0 +/- 14.9%携带Fc受体的细胞和12.9 +/- 4.4%单核细胞和巨噬细胞。虽然T γ细胞占外周血T细胞的13.3 +/- 1.4%，但它们仅占外周血T细胞的6.0 +/- 2.0%。相比之下，T μ细胞占洗脱液中T细胞的44.7 +/- 4.9%，占外周血中T细胞的51.6 +/- 4.4%。牙龈中T γ细胞比例的降低可能表明与T抑制B细胞增殖有关的异常免疫调节形式。
Advanced human periodontitis is considered to be a B-cell lesion, but the cellular infiltrate contains several cell types, the distribution of which has not been determined. This experiment was designed to characterize and identify the immunocompetent cells on histological sections and in eluates from diseased human gingiva. Immunoglobulin-bearing cells were detected on histological sections by direct immunofluorescence with F(ab')2 antisera monospecific for human immunoglobulin G (IgG), IgA, or IgM. Plasma cells predominated in the central portion of the lamina propria, with the proportions positive for IgG, IgA, and IgM accounting for 65.2 +/- 9.5, 11.2 +/- 1.1, and 1.3 +/- 1.1% of the total infiltrating cells, respectively. T lymphocytes, identified by indirect immunofluorescence with monoclonal antibody (Leu-1) against human T cells, accounted for 29.3 +/- 10.0% of the total infiltrated cells. Most of the T cells were located subjacent to the pocket epithelium, but there were a few in the central lamina propria. Similarly, Fc receptor-bearing cells detected by EA rosetting and macrophages and monocytes detected by nonspecific esterase staining with alpha-naphthylbutyrate esterase were also localized to the region immediately subjacent to the pocket epithelium. Infiltrated cells were harvested from minced gingival tissue after digestion with collagenase, hyaluronidase, and DNase. The eluates contained 35.3 +/- 6.0% T lymphocytes, 30.0 +/- 14.9% Fc receptor-bearing cells, and 12.9 +/- 4.4% monocytes and macrophages. Whereas T gamma cells comprised 13.3 +/- 1.4% of peripheral blood T cells, they accounted for only 6.0 +/- 2.0% of the eluate T cells. In contrast, T mu cells accounted for 44.7 +/- 4.9% of the T cells in the eluates and 51.6 +/- 4.4% in the peripheral blood. The decreased proportion of T gamma cells in the gingiva may indicate a form of abnormal immune regulation concerned with T suppression of B-cell proliferation.
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影响因子：
6.7
作者：
Listgarten,MA;Lai,CH;Evian,CI
通讯作者：
Evian,CI
研究单克隆抗体识别的人类 T 淋巴细胞抗原。
DOI：
10.1073/pnas.78.3.1791
发表时间：
1981
影响因子：
11.1
作者：
Engleman,EG;Warnke,R;Fox,RI;Dilley,J;Benike,CJ;Levy,R
通讯作者：
Levy,R
影响因子：
4.6
作者：
Ebersole,JL;Taubman,MA;Smith,DJ;Genco,RJ;Frey,DE
通讯作者：
Frey,DE
活动性系统性红斑狼疮中免疫调节 T 细胞亚群的改变。
DOI：
10.1172/jci109948
发表时间：
1980
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Morimoto,C;Reinherz,EL;Schlossman,SF;Schur,PH;Mills,JA;Steinberg,AD
通讯作者：
Steinberg,AD