Commensal microbiota modulate gene expression in the skin.
Commensal microbiota modulate gene expression in the skin.
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DOI：
10.1186/s40168-018-0404-9
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发表时间：
2018-01-30
期刊：
影响因子：
15.5
通讯作者：
Grice EA
中科院分区：
文献类型：
--
作者：
Meisel JS;Sfyroera G;Bartow-McKenney C;Gimblet C;Bugayev J;Horwinski J;Kim B;Brestoff JR;Tyldsley AS;Zheng Q;Hodkinson BP;Artis D;Grice EA
皮肤中存在复杂的微生物群落，但对它们的生理作用和介导皮肤宿主-微生物相互作用的分子机制知之甚少。在这里，我们分析了在存在和不存在微生物群的情况下饲养的小鼠的皮肤转录组，以阐明微生物群在皮肤中调节的途径和功能的范围。共有2820个基因差异调节微生物定植和丰富的基因本体论（GO）术语相关的宿主免疫反应和表皮分化。天然免疫应答基因和参与细胞因子活性的基因通常响应于微生物群而上调，并且包括编码toll样受体、抗微生物肽、补体级联的基因和参与IL-1家族细胞因子信号传导和T细胞归巢的基因。我们的研究结果还揭示了微生物群在调节表皮分化和发育中的作用，以及表皮分化复合体（EDC）中基因的差异表达。具有相关共表达模式的基因在转录因子Klf 4、AP-1和SP-1的结合位点中富集，所有这些转录因子都是表皮分化的调节因子。最后，我们确定了皮肤和胃肠道共同的微生物调节的转录特征。通过这种基础方法，我们建立了一个重要的资源，用于了解皮肤中微生物介导的基因表达的全基因组意义，并强调微生物组有助于皮肤健康和疾病的前瞻性方式。本文的在线版本（10.1186/s40168-018-0404-9）包含补充材料，可供授权用户使用。
The skin harbors complex communities of resident microorganisms, yet little is known of their physiological roles and the molecular mechanisms that mediate cutaneous host-microbe interactions. Here, we profiled skin transcriptomes of mice reared in the presence and absence of microbiota to elucidate the range of pathways and functions modulated in the skin by the microbiota. A total of 2820 genes were differentially regulated in response to microbial colonization and were enriched in gene ontology (GO) terms related to the host-immune response and epidermal differentiation. Innate immune response genes and genes involved in cytokine activity were generally upregulated in response to microbiota and included genes encoding toll-like receptors, antimicrobial peptides, the complement cascade, and genes involved in IL-1 family cytokine signaling and homing of T cells. Our results also reveal a role for the microbiota in modulating epidermal differentiation and development, with differential expression of genes in the epidermal differentiation complex (EDC). Genes with correlated co-expression patterns were enriched in binding sites for the transcription factors Klf4, AP-1, and SP-1, all implicated as regulators of epidermal differentiation. Finally, we identified transcriptional signatures of microbial regulation common to both the skin and the gastrointestinal tract. With this foundational approach, we establish a critical resource for understanding the genome-wide implications of microbially mediated gene expression in the skin and emphasize prospective ways in which the microbiome contributes to skin health and disease. The online version of this article (10.1186/s40168-018-0404-9) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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DOI：
10.1073/pnas.1307855110
发表时间：
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影响因子：
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作者：
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通讯作者：
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