Propionibacterium acnes strain populations in the human skin microbiome associated with acne.
Propionibacterium acnes strain populations in the human skin microbiome associated with acne.
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与痤疮相关的人皮肤微生物组中的痤疮丙酸杆菌菌株群体。
DOI：
10.1038/jid.2013.21
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发表时间：
2013-09
影响因子：
6.5
通讯作者：
Li, Huiying
中科院分区：
文献类型：
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作者：
Fitz-Gibbon, Sorel;Tomida, Shuta;Chiu, Bor-Han;Lin Nguyen;Du, Christine;Liu, Minghsun;Elashoff, David;Erfe, Marie C.;Loncaric, Anya;Kim, Jenny;Modlin, Robert L.;Miller, Jeff F.;Sodergren, Erica;Craft, Noah;Weinstock, George M.;Li, Huiying
人体皮肤微生物组在皮肤健康和疾病中起着重要作用。然而，在菌株水平上的细菌种群结构和多样性知之甚少。我们通过对49名痤疮患者和52名健康个体的鼻子上的毛囊皮脂腺单位进行采样，在痤疮丙酸杆菌（一种主要的皮肤真菌）的菌株水平和基因组水平上比较了皮肤微生物组。宏基因组分析表明，虽然痤疮丙酸杆菌的相对丰度相似，但两个队列中的菌株群体结构显著不同。某些菌株与痤疮高度相关，其他菌株在健康皮肤中富集。通过对66个以前未报道的痤疮丙酸杆菌菌株进行测序并比较71个痤疮丙酸杆菌基因组，我们确定了与痤疮或健康相关的各种痤疮丙酸杆菌菌株的潜在遗传决定因素。我们的分析表明，获得的DNA序列和细菌免疫元件可能在决定痤疮丙酸杆菌菌株的毒力特性中发挥作用，其中一些可能是未来治疗干预的靶点。这项研究证明了一个以前未报道的范式，可以解释人类疾病的发病机制的大肠杆菌菌株种群。它强调了对人类微生物组进行菌株水平分析以确定细菌在健康和疾病中的作用的重要性。
The human skin microbiome plays important roles in skin health and disease. However, bacterial population structure and diversity at the strain level is poorly understood. We compared the skin microbiome at the strain level and genome level of Propionibacterium acnes, a dominant skin commensal, between 49 acne patients and 52 healthy individuals by sampling the pilosebaceous units on their noses. Metagenomic analysis demonstrated that while the relative abundances of P. acnes were similar, the strain population structures were significantly different in the two cohorts. Certain strains were highly associated with acne and other strains were enriched in healthy skin. By sequencing 66 previously unreported P. acnes strains and comparing 71 P. acnes genomes, we identified potential genetic determinants of various P. acnes strains in association with acne or health. Our analysis suggests that acquired DNA sequences and bacterial immune elements may play roles in determining virulence properties of P. acnes strains and some could be future targets for therapeutic interventions. This study demonstrates a previously unreported paradigm of commensal strain populations that could explain the pathogenesis of human diseases. It underscores the importance of strain level analysis of the human microbiome to define the role of commensals in health and disease.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
168.9
作者：
Humar, D;Datta, V;Nizet, V
通讯作者：
Nizet, V
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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