CHRONIC ENTEROVIRUS INFECTION IN PATIENTS WITH POSTVIRAL FATIGUE SYNDROME
CHRONIC ENTEROVIRUS INFECTION IN PATIENTS WITH POSTVIRAL FATIGUE SYNDROME
复制标题
病毒后疲劳综合征患者的慢性肠道病毒感染
DOI：
10.1016/s0140-6736(88)92722-5
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
J. Mowbray
中科院分区：
文献类型：
--
作者：
G. Yousef;G. Mann;D. Smith;E. Bell;V. Murugesan;R. Mccartney;J. Mowbray
对76例病毒感染后疲劳综合征（PVFS）患者和30例对照组进行了研究。尝试通过直接培养和在病毒与抗体酸解离后从浓缩的粪便样品中分离病毒。肠道病毒培养阳性17例（22%）和2例（7%）对照。一种肠道病毒组特异性单克隆抗体，5-D8/1，直接对VP 1多肽，用于检测肠道病毒抗原在循环中，无论是游离或与抗体复合。在另一组87例PVFS患者中，44例（51%）血清中检测到VP 1抗原。当可检测到IgM复合物时，VP 1抗原阳性的患者数（42/44）高于不可检测时（2/23）。1年后，第一组76例培养阳性的17例患者再次接受研究。5例（29%）再次分离到相同的病毒，13例（76%）血清中可检测到肠道病毒IgM应答，9例（53%）血清中VP 1抗原阳性。这些结果表明，肠道病毒的慢性感染发生在许多PVFS患者和肠道病毒抗原的血清中的检测是一个敏感和令人满意的方法，调查这些患者的感染。
76 patients with the postviral fatigue syndrome (PVFS) and 30 matched controls were investigated. Virus isolation was attempted from concentrated faecal samples by direct culture and after acid dissociation of virus from antibody. Positive cultures of enteroviruses were obtained from 17 (22%) patients and 2 (7%) controls. An enterovirus-group-specific monoclonal antibody, 5-D8/1, directed against the VP1 polypeptide, was used to detect enteroviral antigen in the circulation, either free or complexed with antibody. VP1 antigen was detected in the serum of 44 (51%) of a further group of 87 PVFS patients. The number of patients positive for VP1 antigen was greater (42/44) when IgM complexes were detectable than when they were not (2/23). 1 year later, the 17 patients of the first group of 76 with positive cultures were again studied. The same virus was again isolated from 5 (29%), 13 (76%) had detectable IgM responses to enteroviruses, and 9 (53%) were positive for VP1 antigen in the serum. These results show that chronic infection with enteroviruses occurs in many PVFS patients and that detection of enterovirus antigen in the serum is a sensitive and satisfactory method for investigating infection in these patients.
影响因子：
4.1
作者：
CANN, CE;GENANT, HK;ETTINGER, B
通讯作者：
ETTINGER, B