Neural proliferation and restoration of neurochemical phenotypes and compromised functions following capsaicin-induced neuronal damage in the nodose ganglion of the adult rat.
Neural proliferation and restoration of neurochemical phenotypes and compromised functions following capsaicin-induced neuronal damage in the nodose ganglion of the adult rat.
复制标题
DOI：
10.3389/fnins.2011.00012
复制
发表时间：
2011
影响因子：
4.3
通讯作者：
Czaja K
中科院分区：
文献类型：
--
作者：
Gallaher ZR;Ryu V;Larios RM;Sprunger LK;Czaja K
我们之前报道过，在辣椒素诱导的近一半神经元群的破坏后60天，成人结节神经节（NG）内的神经元数量恢复到正常水平。然而，这种神经元替代的性质尚不清楚。因此，我们旨在描述辣椒素诱导损伤后成年大鼠NG神经元的神经增殖、神经化学表型和功能恢复。Sprague-Dawley大鼠腹腔注射辣椒素或载体溶液，然后注射5-溴-2-脱氧尿苷（BrdU），观察细胞增殖情况。在处理后（长达300天）多次收集NG，并进行免疫荧光、RT-PCR和分散细胞培养。BrdU/ ki -67标记的细胞表明，辣椒素诱导的细胞增殖表明，丢失的神经元通过细胞分裂被取代。NG细胞表达干细胞标记物nestin，表明这些神经节具有产生新神经元的能力。brdu掺入辣椒素后β-III微管蛋白阳性的神经元谱表明增殖细胞成熟成为神经元。在使用辣椒素后180天，NG神经元显示NMDAR表达降低。然而，NMDAR在NG内的表达和突触素在NG神经元中心靶点NTS内的表达在300天后恢复到损伤前水平。辣椒素处理大鼠的NG培养物含有双极神经元，通常只在发育过程中发现。为了检测NG神经元的功能恢复情况，我们注射饱腹感分子CCK。CCK对摄食量的影响在辣椒素作用后300天恢复。这种恢复可能是由于受损的NG神经元的再生或功能性神经元的产生取代了失去的连接。
We previously reported that neuronal numbers within adult nodose ganglia (NG) were restored to normal levels 60 days following the capsaicin-induced destruction of nearly half of the neuronal population. However, the nature of this neuronal replacement is not known. Therefore, we aimed to characterize neural proliferation, neurochemical phenotypes, and functional recovery within adult rat NG neurons following capsaicin-induced damage. Sprague-Dawley rats received intraperitoneal injections of capsaicin or vehicle solution, followed by 5-bromo-2-deoxyuridine (BrdU) injections to reveal cellular proliferation. NG were collected at multiple times post-treatment (up to 300 days) and processed for immunofluorescence, RT-PCR, and dispersed cell cultures. Capsaicin-induced cellular proliferation, indicated by BrdU/Ki-67-labeled cells, suggests that lost neurons were replaced through cell division. NG cells expressed the stem cell marker, nestin, indicating that these ganglia have the capacity to generate new neurons. BrdU-incorporation within β-III tubulin-positive neuronal profiles following capsaicin suggests that proliferating cells matured to become neurons. NG neurons displayed decreased NMDAR expression up to 180-days post-capsaicin. However, both NMDAR expression within the NG and synaptophysin expression within the central target of NG neurons, the NTS, were restored to pre-injury levels by 300 days. NG cultures from capsaicin-treated rats contained bipolar neurons, normally found only during development. To test the functional recovery of NG neurons, we injected the satiety molecule, CCK. The effect of CCK on food intake was restored by 300-days post-capsaicin. This restoration may be due to the regeneration of damaged NG neurons or generation of functional neurons that replaced lost connections.
登录
查看更多内容
影响因子：
13.9
作者：
Kriegstein A;Alvarez-Buylla A
通讯作者：
Alvarez-Buylla A
DOI：
10.1523/jneurosci.1145-09.2009
发表时间：
2009-08-05
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Liu MT;Kuan YH;Wang J;Hen R;Gershon MD
通讯作者：
Gershon MD
影响因子：
2.5
作者：
Gallaher, Zachary R.;Larios, Rose Marie;Czaja, Krzysztof
通讯作者：
Czaja, Krzysztof
影响因子：
2.5
作者：
Czaja, Krzysztof;Ritter, Robert C.;Burns, Gilbert A.
通讯作者：
Burns, Gilbert A.
影响因子：
3.3
作者：
Czaja, K.;Burns, G. A.;Ritter, R. C.
通讯作者：
Ritter, R. C.