Progress in Optimization of Agrobacterium-Mediated Transformation in Sorghum (Sorghum bicolor).
Progress in Optimization of Agrobacterium-Mediated Transformation in Sorghum (Sorghum bicolor).
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农杆菌介导高粱转化优化研究进展
DOI：
10.3390/ijms19102983
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发表时间：
2018-09-29
影响因子：
5.6
通讯作者：
Kong Y
中科院分区：
文献类型：
--
作者：
Ahmed RI;Ding A;Xie M;Kong Y
这篇综述总结了过去二十年取得的成就，并简要概述了影响农杆菌介导的双色高粱转化的一些重要因素。最近，通过直接DNA传递方法和通过农杆菌的间接方法，在这种特殊的单子叶作物的成功转化方面取得了进展。然而，较低的转化率仍然是高粱转基因的瓶颈。通过优化初步测定，可以获得有效的农杆菌转化系统，包括外植体来源、生长培养基、抗生素、农杆菌菌株和愈伤组织的农杆菌感染反应。遗传转化感受态菌株的选择也是考虑的关键因素之一。成功的转化高度依赖于所选品种的基因组配置，其中非单宁基因型被证明是最适合的。来自田间的未成熟胚胎比来自温室的胚胎具有更高的固有适应机会。较高浓度的农杆菌可能会损坏外植体来源。利用抗坏死治疗和优化组织培养时间是降低酚类化合物影响的适当策略。在组织培养的不同阶段适当选择培养基容器也可能有助于建设性的方式。总之，培养基组成、外植体来源和基因型等培养环境对于农杆菌介导的高粱转化系统的成功起着不可或缺的作用。
This review archives the achievements made in the last two decades and presents a brief outline of some significant factors influencing the Agrobacterium-mediated transformation of Sorghum bicolor. Recently, progress in successful transformation has been made for this particular monocot crop through direct DNA delivery method and indirect method via Agrobacterium. However, lower transformation rate still proved to be a bottleneck in genetic modification of sorghum. An efficient Agrobacterium transformation system could be attained by optimizing the preliminary assays, comprising of explant source, growth media, antibiotics, Agrobacterium strains and agro-infection response of callus. The selection of competent strains for genetic transformation is also one of the key factors of consideration. Successful transformation is highly dependent on genome configuration of selected cultivar, where non-tannin genotype proved the best suited. Immature embryos from the field source have higher inherent adaptation chances than that of the greenhouse source. A higher concentration of Agrobacterium may damage the explant source. Utilization of anti-necrotic treatments and optimized tissue culture timeframe are the adequate strategies to lower down the effect of phenolic compounds. Appropriate selection of culture media vessels at different stages of tissue culture may also assist in a constructive manner. In conclusion, some aspects such as culture environment with medium composition, explant sources, and genotypes play an indispensable role in successful Agrobacterium-mediated sorghum transformation system.
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影响因子：
3
作者：
Elkonin, LA;Pakhomova, NV
通讯作者：
Pakhomova, NV
影响因子：
4.3
作者：
Enríquez-Obregón, GA;Vázquez-Padrón, RI;Selman-Housein, G
通讯作者：
Selman-Housein, G
影响因子：
1.1
作者：
ADKINS, SW
通讯作者：
ADKINS, SW
影响因子：
11.6
作者：
Cook, Daniel;Rimando, Agnes M.;Baerson, Scott R.
通讯作者：
Baerson, Scott R.
影响因子：
2.1
作者：
Carvalho, Carlos Henrique S.;Zehr, Usha B.;Axtell, John D.
通讯作者：
Axtell, John D.