Diagnosis of pancreatic ductal adenocarcinoma and chronic pancreatitis by measurement of microRNA abundance in blood and tissue.
Diagnosis of pancreatic ductal adenocarcinoma and chronic pancreatitis by measurement of microRNA abundance in blood and tissue.
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DOI：
10.1371/journal.pone.0034151
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hoheisel JD
中科院分区：
文献类型：
--
作者：
Bauer AS;Keller A;Costello E;Greenhalf W;Bier M;Borries A;Beier M;Neoptolemos J;Büchler M;Werner J;Giese N;Hoheisel JD
一个可靠的诊断过程可能对胰腺癌的成功治疗产生重大影响，目前胰腺癌的死亡率与发病率几乎相同。在微阵列上分析了外周血和胰腺组织中所有microRNA分子丰度的变化，并通过实时PCR分析进行了部分验证。总共研究了来自两个临床中心的245个样本，这些样本来自胰腺导管腺癌或慢性胰腺炎患者和健康供体。利用微创血液检查，受试者工作特征（ROC）曲线和相应的曲线下面积（AUC）分析表明，在区分健康人群和癌症或慢性胰腺炎患者方面具有非常高的敏感性和特异性；AUC分别为0.973和0.950。对于AUC值分别为1.0和0.937的组织样品，可以进行确证性诊断，部分甚至更具判别性的诊断。此外，还可以区分癌症和慢性胰腺炎（AUC = 0.875）。此外，一些mirna在组织和血液样本中都表现出丰度变化。该结果对于评估接受根治性手术切除的患者以及具有家族性胰腺癌风险的患者的肿瘤复发具有直接的诊断价值。
A solid process for diagnosis could have a substantial impact on the successful treatment of pancreatic cancer, for which currently mortality is nearly identical to incidence. Variations in the abundance of all microRNA molecules from peripheral blood cells and pancreas tissues were analyzed on microarrays and in part validated by real-time PCR assays. In total, 245 samples from two clinical centers were studied that were obtained from patients with pancreatic ductal adenocarcinoma or chronic pancreatitis and from healthy donors. Utilizing the minimally invasive blood test, receiver operating characteristic (ROC) curves and the corresponding area under the curve (AUC) analysis demonstrated very high sensitivity and specificity of a distinction between healthy people and patients with either cancer or chronic pancreatitis; respective AUC values of 0.973 and 0.950 were obtained. Confirmative and partly even more discriminative diagnosis could be performed on tissue samples with AUC values of 1.0 and 0.937, respectively. In addition, discrimination between cancer and chronic pancreatitis was achieved (AUC = 0.875). Also, several miRNAs were identified that exhibited abundance variations in both tissue and blood samples. The results could have an immediate diagnostic value for the evaluation of tumor reoccurrence in patients, who have undergone curative surgical resection, and for people with a familial risk of pancreatic cancer.
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DOI：
10.1074/mcp.m900419-mcp200
发表时间：
2010-06
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Schröder C;Jacob A;Tonack S;Radon TP;Sill M;Zucknick M;Rüffer S;Costello E;Neoptolemos JP;Crnogorac-Jurcevic T;Bauer A;Fellenberg K;Hoheisel JD
通讯作者：
Hoheisel JD
影响因子：
6.4
作者：
Lee, Eun Jon;Gusev, Yuriy;Schmittgen, Thomas D.
通讯作者：
Schmittgen, Thomas D.
影响因子：
--
作者：
SHANNON, CE
通讯作者：
SHANNON, CE
Mirbase：MicroRNA基因组学的工具。
影响因子：
14.9
作者：
Griffiths-Jones, Sam;Saini, Harpreet Kaur;van Dongen, Stijn;Enright, Anton J.
通讯作者：
Enright, Anton J.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y