Microinjection of DLH into the region of the caudal ventral respiratory column in the cat: evidence for an endogenous cough-suppressant mechanism.
Microinjection of DLH into the region of the caudal ventral respiratory column in the cat: evidence for an endogenous cough-suppressant mechanism.
复制标题
将 DLH 显微注射到猫尾部腹侧呼吸柱区域：内源性止咳机制的证据。
DOI：
10.1152/japplphysiol.00616.2006
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Bolser,DonaldC
中科院分区：
文献类型：
--
作者：
Poliacek,Ivan;Corrie,LuWen-Chi;Wang,Cheng;Rose,MelanieJ;Bolser,DonaldC
尾侧腹侧呼吸柱（cVRC）包含运动前呼气神经元，它们在胸壁和腹肌的咳嗽相关呼气活动中发挥重要作用。用微量注射d，l-同型半胱氨酸（DLH）来验证cVRC神经元的局部激活可以抑制咳嗽反射的假设。将DLH（20-50 mM，10-30 nl）注射到9只麻醉的自主呼吸猫的cVRC区域。重复性咳嗽是由胸内气道的机械刺激引起的。肌电图（EMG）记录双侧吸气胸骨旁和呼气腹横肌（ABD）和单侧喉后环杓肌和甲杓肌。单侧微量注射DLH（1- 1.5nmol）可引起双侧紧张性和时相性呼吸ABD肌电活动增加，并改变呼吸模式和喉运动活动。然而，与对照组相比，DLH也使咳嗽频率降低了51 ± 7%（P< 0.001），并且与对照组相比，注射后咳嗽期间对侧（−35 ± 3%;P< 0.001）和同侧（−34 ± 5%;P< 0.001）ABD EMG的振幅也降低了。微量注射低剂量DLH（0.34-0.8 nmol）后，咳嗽改变不明显。微量注射溶媒后未观察到咳嗽抑制。这些结果表明，在cVRC区域可能存在内源性咳嗽抑制神经元网络，并且该网络可能参与咳嗽反射兴奋性的控制。
The caudal ventral respiratory column (cVRC) contains premotor expiratory neurons that play an important role in cough-related expiratory activity of chest wall and abdominal muscles. Microinjection ofd,l-homocysteic acid (DLH) was used to test the hypothesis that local activation of cVRC neurons can suppress the cough reflex. DLH (20–50 mM, 10–30 nl) was injected into the region of cVRC in nine anesthetized spontaneously breathing cats. Repetitive coughing was elicited by mechanical stimulation of the intrathoracic airways. Electromyograms (EMG) were recorded bilaterally from inspiratory parasternal and expiratory transversus abdominis (ABD) and unilaterally from laryngeal posterior cricoarytenoid and thyroarytenoid muscles. Unilateral microinjection of DLH (1–1.5 nmol) elicited bilateral increases in tonic and phasic respiratory ABD EMG activity, and it altered the respiratory pattern and laryngeal motor activities. However, DLH also decreased cough frequency by 51 ± 7% compared with control (P< 0.001) and the amplitude of the contralateral (−35 ± 3%;P< 0.001) and ipsilateral (−34 ± 5%;P< 0.001) ABD EMGs during postinjection coughs compared with control. The cough alterations were much less pronounced after microinjection of a lower dose of DLH (0.34–0.8 nmol). No cough depression was observed after microinjections of vehicle. These results suggest that an endogenous cough suppressant neuronal network in the region of the cVRC may exist, and this network may be involved in the control of cough reflex excitability.
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DOI：
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期刊：
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DOI：
10.1016/s0006-291x(85)80179-0
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