Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos
Involvement of Oct4‐type transcription factor Pou5f3 in posterior spinal cord formation in zebrafish embryos
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Oct4型转录因子Pou5f3参与斑马鱼胚胎后脊髓形成
DOI：
10.1111/dgd.12742
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Yamasu Kyo
中科院分区：
文献类型：
--
作者：
Yuikawa Tatsuya;Ikeda Masaaki;Tsuda Sachiko;Saito Shinji;Yamasu Kyo
在脊椎动物胚胎发生中，后部体的伸长是由轴向和近轴中胚层以及后部末端的神经管的重新产生驱动的。这一过程被认为依赖于尾芽区域的干细胞样群体，但涉及的基因调控网络的细节尚不清楚。先前的研究表明，pou 5 f3，一个Oct 4型POU基因在斑马鱼，参与轴向伸长。在本研究中，我们首次发现pou 5 f3主要在原肠胚形成后立即在尾芽的背侧区域表达，并且这种表达在体节发生期间仅限于伸长神经管的最后区域。这种pou 5 f3的表达与神经管中sox 3的广泛表达互补，并与尾芽中tbxta（T/Brachyury的直向同源物）的特异性表达形成了一个尖锐的边界，暗示pou 5 f3参与了尾芽衍生细胞向脊髓神经分化的特化。当pou 5 f3在原肠胚形成后通过显性干扰pou 5 f3突变基因（en-pou 5 f3）的诱导而功能受损时，躯干和尾巴的伸长在沿着轴的不同位置受到明显干扰，这取决于阶段。这一发现表明pou 5 f3参与了尾芽体的从头生成。有条件的功能废除也表明pou 5 f3下调中胚层形成基因，但通过激活尾芽周围的神经发生基因促进神经发育。提示pou 5 f3参与了脊髓后角的形成。
In vertebrate embryogenesis, elongation of the posterior body is driven by de novo production of the axial and paraxial mesoderm as well as the neural tube at the posterior end. This process is presumed to depend on the stem cell‐like population in the tail bud region, but the details of the gene regulatory network involved are unknown. Previous studies suggested the involvement ofpou5f3, anOct4‐type POU gene in zebrafish, in axial elongation. In the present study, we first found thatpou5f3is expressed mainly in the dorsal region of the tail bud immediately after gastrulation, and that this expression is restricted to the posterior‐most region of the elongating neural tube during somitogenesis. Thispou5f3expression was complementary to the broad expression ofsox3in the neural tube, and formed a sharp boundary with specific expression oftbxta(orthologue of mammalianT/Brachyury) in the tail bud, implicatingpou5f3in the specification of tail bud‐derived cells toward neural differentiation in the spinal cord. Whenpou5f3was functionally impaired after gastrulation by induction of a dominant‐interferingpou5f3mutant gene (en‐pou5f3), trunk and tail elongation were markedly disturbed at distinct positions along the axis depending on the stage. This finding showed involvement ofpou5f3in de novo generation of the body from the tail bud. Conditional functional abrogation also showed thatpou5f3downregulates mesoderm‐forming genes but promotes neural development by activating neurogenesis genes around the tail bud. These results suggest thatpou5f3is involved in formation of the posterior spinal cord.
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影响因子：
--
作者：
Kimelman D
通讯作者：
Kimelman D
影响因子：
4.6
作者：
Vu H. Nguyen;Jamie Trout;Stephanie A. Connors;P. Andermann;Eric Weinberg;Mary C. Mullins
通讯作者：
Vu H. Nguyen;Jamie Trout;Stephanie A. Connors;P. Andermann;Eric Weinberg;Mary C. Mullins
影响因子：
2.7
作者：
Rogers, Crystal D.;Archer, Tenley C.;Casey, Elena M. Silva
通讯作者：
Casey, Elena M. Silva
影响因子：
4.6
作者：
Morrison, GM;Brickman, JM
通讯作者：
Brickman, JM
DOI：
10.1242/dev.164319
发表时间：
2018-10-02
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Rodrigo Albors A;Halley PA;Storey KG
通讯作者：
Storey KG