Coexpression of α-sarcomeric actin, α-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
Coexpression of α-sarcomeric actin, α-smooth muscle actin and desmin during myogenesis in rat and mouse embryos I. Skeletal muscle
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大鼠和小鼠胚胎肌发生过程中 α-肌节肌动蛋白、α-平滑肌肌动蛋白和结蛋白的共表达 I. 骨骼肌
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
G. Gabbiani
中科院分区：
文献类型：
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作者：
F. Babai;J. Musevi;W. Schürch;A. Royal;G. Gabbiani
摘要 采用免疫组织化学方法检测大鼠和小鼠胚胎组织中波形蛋白、结蛋白、α-肌节和 α-平滑肌肌动蛋白的表达。结果显示，在小鼠胎儿胚胎发育过程中，骨骼肌细胞中结蛋白和 α-肌动蛋白亚型（α-sr 和 α-sm）的表达具有相似性。在这两个物种中，在成肌细胞、肌节成肌细胞和肌管中观察到α-sr和α-sm肌动蛋白的共表达。 α-sm 肌动蛋白表达强度在肌发生的末期降低，并在成熟心肌细胞和肌纤维中完全消失。结蛋白在所有预融合成肌细胞（1 型和 2 型成肌细胞）、肌管和肌纤维中表达。体节成肌细胞中结蛋白的出现先于 α-肌动蛋白（α-sr 和 α-sm）的表达几个小时。我们对波形蛋白表达的研究，仅限于大鼠胚胎，表明体节前成肌细胞仅表达波形蛋白，1型成肌细胞表达波形蛋白和结蛋白，2型成肌细胞（横纹肌母细胞）表达结蛋白和α-肌动蛋白（α-sr和α-sm）。我们的研究证明了胎儿胚胎肌发生和肌源性肿瘤分化之间的相似性：结蛋白出现在胚胎成肌细胞中的α-肌动蛋白之前，并且可以被认为是肌源性分化初始步骤的标志物，α-sm肌动蛋白被认为是横纹肌细胞胎儿胚胎肌动蛋白，在发育过程中在骨骼肌母细胞和心肌母细胞中短暂表达，并在骨骼肿瘤转化过程中重新出现。肌肉。
Abstract Expression of vimentin, desmin, α-sarcomeric and α-smooth muscle actins in embryonic tissues of rat and mice was examined using an immunohistochemical approach. The results showed a similarity in the expression of desmin and α-actin isoforms (α-sr and α-sm) in skeletal muscle cells during murine feto-embryonic development. In the two species, coexpression of α-sr and α-sm actins has been observed in cardiomyoblasts, myotomal myoblasts and myotubes. The intensity of α-sm actin expression decreased during the terminal steps of myogenesis and disappeared completely in mature cardiomyocytes and myofibres. Desmin was expressed in all prefusion myoblasts (type 1 and 2 myoblasts), myotubes, and in myofibres. The appearance of desmin in myoblasts of somites preceded by a few hours the expression of the α-actins (α-sr and α-sm). Our study on vimentin expression, limited to rat embryos, revealed that somite premyoblasts expressed only vimentin, type 1 myoblasts expressed vimentin and desmin, and type 2 myoblasts (rhabdomyoblasts) expressed desmin and α-actins (α-sr and α-sm). Our study demonstrates the resemblance between feto-embryonic myogenesis and myogenic neoplastic differentiation: desmin appears before the α-actins in embryonic myoblasts, and can be considered as a marker of an initial step in myogenic differentiation, α-sm actin, considered as a striated muscle cell feto-embryonic actin, is expressed transiently in skeletal myoblasts and cardiomyoblasts during development and reappears during neoplastic transformation of skeletal muscle.
发育中的小鸡脊髓中的中间丝蛋白。
影响因子：
2.7
作者：
Tapscott,SJ;Bennett,GS;Toyama,Y;Kleinbart,F;Holtzer,H
通讯作者：
Holtzer,H
佛波醇12-肉豆蔻酸酯13-乙酸酯对胚胎鸡骨骼肌成肌细胞分化程序的影响。
影响因子：
11.2
作者：
Dlugosz,AA;Tapscott,SJ;Holtzer,H
通讯作者：
Holtzer,H