Curcumin Efficacy in a Serum/Glucose Deprivation-Induced Neuronal PC12 Injury Model.
Curcumin Efficacy in a Serum/Glucose Deprivation-Induced Neuronal PC12 Injury Model.
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DOI：
10.2174/1874467214666210203211312
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发表时间：
2021
影响因子：
2.7
通讯作者：
Samarghandian S
中科院分区：
文献类型：
--
作者：
Farkhondeh T;Ashrafizadeh M;Azimi-Nezhad M;Samini F;Aschner M;Samarghandian S
葡萄糖/血清剥夺（GSD）已被用于了解缺血期间神经元损伤的分子机制。有人认为姜黄素可以改善神经退行性疾病。在这项研究中，姜黄素的保护作用和其潜在的机制进行了研究，在PC 12细胞对GSD诱导的压力。将PC 12细胞在DMEM中培养过夜，然后在GSD条件下孵育6或12小时。GSD处理的细胞用不同浓度的姜黄素（10、20和40 μM）预处理5小时。测定细胞活力、凋亡、活性氧（ROS）水平、氧化应激、凋亡相关基因表达和IL-6。姜黄素在GSD作用12小时后可提高PC 12细胞的存活率并引起抗凋亡作用。姜黄素还增加抗氧化酶的表达，抑制脂质过氧化反应，并减少白细胞介素-6分泌的PC 12细胞进行GSD。此外，姜黄素预处理下调促凋亡（Bax）和上调抗凋亡（Bcl 2）介质。姜黄素减轻缺血的许多不良反应，因此，应被视为缺血患者的辅助治疗。
Glucose/serum deprivation (GSD), has been used for understanding molecular mechanisms of neuronal damage during ischemia. It has been suggested that curcumin may improve neurodegenerative diseases. In this study, the protective effects of curcumin and its underlying mechanisms were investigated in PC12 cells upon GSD-induced stress. PC12 cells were cultured in DMEM overnight and then incubated in GSD condition for either 6 or 12h. GSD-treated cells were pretreated with various concentrations of curcumin (10, 20, and 40 μM) for 5h. The cell viability, apoptosis, reactive oxygen species (ROS) level, oxidative stress, expression of apoptosis-related genes, and IL-6 were determined. Curcumin increased cell viability and caused an anti-apoptotic effect in PC12 cells exposed for 12h to GSD . Curcumin also increased antioxidant enzyme expression, suppressed lipid peroxidation, and decreased interleukin-6 secretion in PC12 cells subjected to GSD. In addition, pretreatment with curcumin down-regulated pro-apoptotic (Bax), and up-regulated antiapoptotic (Bcl2) mediators. Curcumin mitigates many of the adverse effects of ischemia, and therefore, should be considered as an adjunct therapy in ischemic patients.
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影响因子：
4.4
作者：
Li, Li;Du, Jikun;Lee, Yongwoo
通讯作者：
Lee, Yongwoo
影响因子：
6.1
作者：
Izuta, Hiroshi;Shimazawa, Masamitsu;Hara, Hideaki
通讯作者：
Hara, Hideaki
影响因子：
5.7
作者：
Lao, CJ;Lin, JG;Hsieh, CL
通讯作者：
Hsieh, CL
姜黄素的神经保护作用与脑缺血再灌注损伤中自噬和HIF-1α相互作用的调节有关
影响因子：
4.8
作者：
Hou, Yang;Wang, Jue;Feng, Juan
通讯作者：
Feng, Juan
DOI：
10.1016/j.bbamcr.2014.11.009
发表时间：
2015-02-01
影响因子：
5.1
作者：
Lahiani, Adi;Zahavi, Efrat;Lazarovici, Philip
通讯作者：
Lazarovici, Philip