Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
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人参皂苷 Rg1 在体外以剂量依赖性方式加速人乳腺脂肪干细胞的旁分泌活性和脂肪形成分化
DOI：
10.1177/0963689719825615
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发表时间：
2019-01
影响因子：
3.3
通讯作者：
黎洪棉
中科院分区：
文献类型：
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作者：
梁至洁;陆翔;朱丹丹;易晓林;吴芳晓;何宁;唐超;韦长元;黎洪棉
增强脂肪来源的基质细胞（ASCs）的生物学功能是再生医学中促进组织重塑的一种有前景的方法。在此，我们研究了人参皂苷Rg1对旁分泌活性和脂肪生成分化的影响。
Augmenting the biological function of adipose-derived stromal cells (ASCs) is a promising approach to promoting tissue remodeling in regenerative medicine. Here, we examined the effect of ginsenoside Rg1 on the paracrine activity and adipogenic differentiation capacity of human breast ASCs (hbASCs) in vitro. hbASCs were isolated and characterized in terms of stromal cell surface markers and multipotency. Third-passage hbASCs were cultured in basic media only or basic media containing different concentrations of G-Rg1 (0.1–100 μM). Cell proliferation was assessed by CCK-8 assay. Paracrine activity was assessed using ELISA. Gene expression was measured by qRT-PCR. Adipogenic differentiation capacity was evaluated by Oil red O staining. We found that hbASCs differentiated into adipocytes, osteoblasts, and chondrocytes in appropriate induction culture medium. hbASCs showed expression of CD29, CD44, CD49d, CD73, CD90, CD105, and CD133 but not CD31 and CD45 surface markers. G-Rg1 increased hbASC proliferation and adipogenic differentiation capacity at lower concentrations (0.1–1 μM) and had the opposite effects at higher concentrations (10–100 μM), while enhanced paracrine activity was observed in all experimental groups compared with control group, and the activation effect of lower concentration G-Rg1 was greater than at higher concentration. These results indicate that G-Rg1 can enhance the proliferation, paracrine activity, and adipogenic differentiation capacity of hbASCs within a certain concentration range. Therefore, the use of G-Rg1 may be beneficial to ASC-assisted fat graft regeneration and soft tissue engineering.
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影响因子：
3.6
作者：
Binzhi Tang;Dejian Wang;Mao-jun Li;Qing Wu;Qian Yang;Wei Shi;Chang-hui Chen
通讯作者：
Binzhi Tang;Dejian Wang;Mao-jun Li;Qing Wu;Qian Yang;Wei Shi;Chang-hui Chen
影响因子：
--
作者：
Fang-tian Xu;Hong-Mian Li;Q. Yin;Da-lie Liu;Hua Nan;Pei-ran Zhao;Shuang-wu Liang
通讯作者：
Fang-tian Xu;Hong-Mian Li;Q. Yin;Da-lie Liu;Hua Nan;Pei-ran Zhao;Shuang-wu Liang
影响因子：
3.6
作者：
Jun Zhang;X. Bai;Bin Zhao;Yunchuan Wang;L. Su;Peng Chang;Xujie Wang;Shichao Han;Jianxin Gao;Xiao-long Hu;D. Hu;Xiaoyan Liu
通讯作者：
Jun Zhang;X. Bai;Bin Zhao;Yunchuan Wang;L. Su;Peng Chang;Xujie Wang;Shichao Han;Jianxin Gao;Xiao-long Hu;D. Hu;Xiaoyan Liu
影响因子：
2.4
作者：
ILLOUZ, YG
通讯作者：
ILLOUZ, YG
通过不同体积分数的富含血小板的血浆和脂肪干细胞提高脂肪移植物的存活率。
影响因子：
2.9
作者：
Li, Feng;Guo, Weihua;Tian, Weidong
通讯作者：
Tian, Weidong