MrTPS3 and MrTPS20 Are Responsible for β-Caryophyllene and α-Pinene Production, Respectively, in Red Bayberry (Morella rubra).
MrTPS3 and MrTPS20 Are Responsible for β-Caryophyllene and α-Pinene Production, Respectively, in Red Bayberry (Morella rubra).
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DOI：
10.3389/fpls.2021.798086
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发表时间：
2021
影响因子：
5.6
通讯作者：
Gao Z
中科院分区：
文献类型：
--
作者：
Wang Y;Yang Q;Zhu Y;Zhao L;Ju P;Wang G;Zhou C;Zhu C;Jia H;Jiao Y;Jia H;Gao Z
杨梅是一种甜而酸的水果，原产于中国，在南方广泛种植。形成杨梅独特香气的关键有机化合物是萜类化合物，主要是β-香芹烯和α-蒎烯。然而，负责不同萜类化合物的关键基因仍然未知。在这里，对果实发育期间具有不同萜类化合物产生的四个品种的样品进行转录组分析，提供了挥发性有机化合物（VOC）产生的候选基因。萜烯脱氢酶（TPS）是调控萜类化合物生物合成的关键酶，在杨梅基因组中已鉴定出34个TPS家族成员。qRT-PCR结果表明，2号染色体上的MrTPS 3和7号染色体上的MrTPS 20分别是调控β-carbellene和α-pinene合成的关键基因。亚细胞定位和酶活性测定结果表明，MrTPS 3和MrTPS 20分别负责β-carbitallene（倍半萜）和α-pinene（单萜）的合成。值得注意的是，深色品种和浅色品种之间的一个氨基酸替换导致MrTPS 3功能的丧失，从而导致不同的β-卡隆烯产生。本研究结果为杨梅挥发性有机化合物的研究奠定了基础，并为杨梅的分子育种提供了潜在的基因。
Red bayberry is a sweet, tart fruit native to China and grown widely in the south. The key organic compounds forming the distinctive aroma in red bayberry, are terpenoids, mainly β-caryophyllene and α-pinene. However, the key genes responsible for different terpenoids are still unknown. Here, transcriptome analysis on samples from four cultivars, during fruit development, with different terpenoid production, provided candidate genes for volatile organic compound (VOC) production. Terpene synthases (TPS) are key enzymes regulating terpenoid biosynthesis, and 34 TPS family members were identified in the red bayberry genome. MrTPS3 in chromosome 2 and MrTPS20 in chromosome 7 were identified as key genes regulating β-caryophyllene and α-pinene synthesis, respectively, by qRT-PCR. Subcellular localization and enzyme activity assay showed that MrTPS3 was responsible for β-caryophyllene (sesquiterpenes) production and MrTPS20 for α-pinene (monoterpenes). Notably, one amino acid substitution between dark color cultivars and light color cultivars resulted in the loss of function of MrTPS3, causing the different β-caryophyllene production. Our results lay the foundation to study volatile organic compounds (VOCs) in red bayberry and provide potential genes for molecular breeding.
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