Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells.
Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells.
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DOI：
10.1016/j.neuron.2021.10.036
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发表时间：
2022-02-02
期刊：
影响因子：
16.2
通讯作者：
Sestan N
中科院分区：
文献类型：
--
作者：
Franjic D;Skarica M;Ma S;Arellano JI;Tebbenkamp ATN;Choi J;Xu C;Li Q;Morozov YM;Andrijevic D;Vrselja Z;Spajic A;Santpere G;Li M;Zhang S;Liu Y;Spurrier J;Zhang L;Gudelj I;Rapan L;Takahashi H;Huttner A;Fan R;Strittmatter SM;Sousa AMM;Rakic P;Sestan N
中枢-内嗅系统支持认知功能，在许多物种中具有终身的神经原性能力，并且选择性地易受阿尔茨海默病的影响。为了研究神经发生潜力和细胞多样性，我们分析了人类、猕猴和猪的5个前额叶-内嗅亚区的单核转录组。综合跨物种分析显示，强大的转录组学和组织学特征的神经发生在成年小鼠，猪和猕猴，但不是人类。双皮质素（DCX）是一种被广泛接受的新生成颗粒细胞的标志物，在不同的人类神经元中检测到，但它并没有定义未成熟的神经元群体。为了探索细胞多样性的物种差异和对疾病的影响，我们表征了亚区域特异性转录组学定义的细胞类型和从三层原始皮层到六层新皮层的过渡变化。值得注意的是，胃L7 B定义了灵长类动物中的亚区域特异性兴奋性神经元和星形胶质细胞，与内质网和脂滴蛋白（包括阿尔茨海默病相关蛋白）相关。这些资源共同揭示了细胞类型和物种特异性的特性，这些特性塑造了大脑内嗅神经发生和功能。利用成年人、猕猴和猪的鼻-内嗅系统的snRNA-seq，Franjic等人定义了共有和不同的细胞类型特征，如在一些兴奋性神经元和星形胶质细胞中的灵长类动物特异性表达胃L7 B。他们还在小鼠、猪和猕猴中确定了神经发生的强大转录组学和组织学特征，但在人类中没有。
The hippocampal-entorhinal system supports cognitive functions, has lifelong neurogenic capabilities in many species, and is selectively vulnerable to Alzheimer’s disease. To investigate neurogenic potential and cellular diversity, we profiled single-nucleus transcriptomes in five hippocampal-entorhinal subregions in human, macaque, and pig. Integrated cross-species analysis revealed robust transcriptomic and histologic signatures of neurogenesis in adult mouse, pig and macaque, but not humans. Doublecortin (DCX), a widely accepted marker of newly generated granule cells, was detected in diverse human neurons, but it did not define immature neuron populations. To explore species differences in cellular diversity and implications for disease, we characterized subregion-specific transcriptomically-defined cell types and transitional changes from the three-layered archicortex to the six-layered neocortex. Notably, METTL7B defined subregion-specific excitatory neurons and astrocytes in primates, associated with endoplasmic reticulum and lipid droplet proteins, including Alzheimer’s disease-related proteins. Together this resource reveals cell-type- and species-specific properties shaping hippocampal-entorhinal neurogenesis and function. Using snRNA-seq of the adult human, macaque and pig hippocampal-entorhinal system, Franjic et al. defined shared and divergent cell type features, like primate-specific expression of METTL7B in some excitatory neurons and astrocytes. They also identified robust transcriptomic and histologic signatures of neurogenesis in mouse, pig and macaque, but not human.
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
64.5
作者：
Bonaguidi MA;Wheeler MA;Shapiro JS;Stadel RP;Sun GJ;Ming GL;Song H
通讯作者：
Song H
影响因子：
5.4
作者：
Dull, T;Zufferey, R;Naldini, L
通讯作者：
Naldini, L
影响因子：
5
作者：
Dennis CV;Suh LS;Rodriguez ML;Kril JJ;Sutherland GT
通讯作者：
Sutherland GT
DOI：
10.1073/pnas.0804558105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Breunig, Joshua J.;Sarkisian, Matthew R.;Town, Terrence
通讯作者：
Town, Terrence