Human adult neurogenesis across the ages: An immunohistochemical study.
Human adult neurogenesis across the ages: An immunohistochemical study.
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DOI：
10.1111/nan.12337
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发表时间：
2016-12
影响因子：
5
通讯作者：
Sutherland GT
中科院分区：
文献类型：
--
作者：
Dennis CV;Suh LS;Rodriguez ML;Kril JJ;Sutherland GT
出生后人脑中的神经发生发生在两个神经发生龛中：侧脑室壁的脑室下区（SVZ）和海马的颗粒下区（SGZ）。这一生理过程持续到成年的程度是一个正在进行的研究领域。本研究的目的是检测细胞增殖的标志物，并评估用于识别人脑两个神经原性小生境中神经发生的抗体的功效。采用免疫组织化学和免疫荧光结合无偏体视学方法，同时检测了23名0.2-59岁个体SVZ和SGZ的细胞增殖和神经发生。有一个显着的下降，增殖细胞在两个神经源性龛在婴儿早期的水平达到那些在相邻的实质中看到的4岁和1岁，在SVZ和SGZ，分别。此外，两个龛中这些增殖细胞的表型都随着年龄的增长而变化。在婴儿中，增殖细胞共表达神经祖细胞（表皮生长因子受体），未成熟神经元（doublecortin和β III微管蛋白）和少突胶质细胞（Olig 2）标记物。然而，三岁后，小胶质细胞是唯一的增殖细胞中发现的壁龛或在相邻的实质。这项研究表明，在儿童早期的两个神经原性龛的神经发生显着下降，并在成人大脑中的稀疏增殖细胞主要是小胶质细胞。
Neurogenesis in the postnatal human brain occurs in two neurogenic niches; the subventricular zone (SVZ) in the wall of the lateral ventricles and the subgranular zone of the hippocampus (SGZ). The extent to which this physiological process continues into adulthood is an area of ongoing research. This study aimed to characterise markers of cell proliferation and assess the efficacy of antibodies used to identify neurogenesis in both neurogenic niches of the human brain. Cell proliferation and neurogenesis were simultaneously examined in the SVZ and SGZ of 23 individuals aged 0.2–59 years using immunohistochemistry and immunofluorescence in combination with unbiased stereology. There was a marked decline in proliferating cells in both neurogenic niches in early infancy with levels reaching those seen in the adjacent parenchyma by four and one year of age, in the SVZ and SGZ, respectively. Furthermore, the phenotype of these proliferating cells in both niches changed with age. In infants, proliferating cells co-expressed neural progenitor (epidermal growth factor receptor), immature neuronal (doublecortin and beta III tubulin) and oligodendrocytic (Olig2) markers. However, after three years of age, microglia were the only proliferating cells found in either niche or in the adjacent parenchyma. This study demonstrates a marked decline in neurogenesis in both neurogenic niches in early childhood, and that the sparse proliferating cells in the adult brain are largely microglia.
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影响因子：
3.4
作者：
Jabes, Adeline;Lavenex, Pamela Banta;Lavenex, Pierre
通讯作者：
Lavenex, Pierre
影响因子：
3.4
作者：
Couillard-Despres, S;Winner, B;Aigner, L
通讯作者：
Aigner, L
影响因子：
16.2
作者：
Bergmann, Olaf;Liebl, Jakob;Frisen, Jonas
通讯作者：
Frisen, Jonas
影响因子：
3.7
作者：
Klempin F;Kronenberg G;Cheung G;Kettenmann H;Kempermann G
通讯作者：
Kempermann G
DOI：
10.1016/s0169-328x(98)00040-0
发表时间：
1998-06-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Ito, D;Imai, Y;Kohsaka, S
通讯作者：
Kohsaka, S