Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.
Identification of host genes that affect acquisition of an integrative and conjugative element in Bacillus subtilis.
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DOI：
10.1111/mmi.12736
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发表时间：
2014-09
影响因子：
3.6
通讯作者：
Grossman AD
中科院分区：
文献类型：
--
作者：
Johnson CM;Grossman AD
接合是细菌中水平基因转移的一种主要类型，涉及使用供体编码的接合机制将DNA从供体转移到受体。使用高通量筛选（Tn-seq），我们确定了受体中有助于枯草芽孢杆菌获得整合和接合元件ICEBs 1的基因。我们发现，一些基因的无效突变导致结合效率增加，而另一些基因则导致结合效率降低。一些突变仅在受体中存在时才影响接合。当存在于供体或受体中时，其他突变影响缀合。大多数被鉴定的基因是已知的或预测会影响细胞被膜。几个编码参与磷脂生物合成的酶，一个编码青霉素结合蛋白的同系物。鉴定的两个基因也影响Tn 916的接合，表明它们在接合中的作用可能是普遍的。我们没有在受体中鉴定出任何对ICEBs 1结合至关重要的基因，这表明如果有这样的基因，那么这些基因要么对细胞生长至关重要，要么是多余的。我们的研究结果表明，收购ICEBs 1，也许其他共轭元素，是强大的，不容易避免的突变和几个膜相关的功能影响共轭的效率。
Conjugation, a major type of horizontal gene transfer in bacteria, involves transfer of DNA from a donor to a recipient using donor-encoded conjugation machinery. Using a high throughput screen (Tn-seq), we identified genes in recipients that contribute to acquisition of the integrative and conjugative element ICEBs1 by Bacillus subtilis. We found that null mutations in some genes caused an increase, and others a decrease in conjugation efficiency. Some mutations affected conjugation only when present in recipients. Other mutations affected conjugation when present in donors or recipients. Most of the genes identified are known or predicted to affect the cell envelope. Several encode enzymes involved in phospholipid biosynthesis and one encodes a homolog of penicillin binding proteins. Two of the genes identified also affected conjugation of Tn916, indicating that their roles in conjugation may be general. We did not identify any genes in recipients that were essential for ICEBs1 conjugation, indicating that if there are such genes, then these are either essential for cell growth or redundant. Our results indicate that acquisition of ICEBs1, and perhaps other conjugative elements, is robust and not easily avoided by mutation and that several membrane-related functions affect the efficiency of conjugation.
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影响因子：
4.5
作者：
Guglielmini J;Quintais L;Garcillán-Barcia MP;de la Cruz F;Rocha EP
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影响因子：
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作者：
FOSTER, SJ
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3.2
作者：
Berkmen, Melanie B.;Lee, Catherine A.;Grossman, Alan D.
通讯作者：
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