Effects of chiral herbicide dichlorprop on Arabidopsis thaliana metabolic profile and its implications for microbial communities in the phyllosphere
Effects of chiral herbicide dichlorprop on Arabidopsis thaliana metabolic profile and its implications for microbial communities in the phyllosphere
复制标题
手性除草剂敌敌草对拟南芥代谢谱的影响及其对叶际微生物群落的影响
DOI：
10.1007/s11356-021-17936-y
复制
发表时间：
2022-01
影响因子：
5.8
通讯作者：
Qian Haifeng
中科院分区：
文献类型：
--
作者：
Qiu Danyan;Ye Yizhi;Ke Mingjing;Xu Nuohan;Zhang Zhenyan;Zhang Fan;Kang Jian;Yu Yitian;Lu Tao;Qian Haifeng
2-(2，4-二氯苯氧基)丙酸(DCPP)是一种常用的除草剂，在土壤中可检出残留。目前尚不清楚手性DCPP是否对植物新陈代谢和叶圈微生物群落产生对映选择性不利影响。本研究以拟南芥为模式植物，探讨R-和S对映体对植物生理活性、叶际微生物群落代谢及相关变化的影响。结果表明，R-DCPP处理后植株鲜重下降了37.6%，S-DCPP处理后植株鲜重增加了7.6%。与S-DCPP相比，R-DCPP对叶片形态、叶肉细胞结构和叶片代谢产物的干扰作用更强。GC-MS分析表明，S-DCPP和R-DCPP处理的拟南芥叶片在碳水化合物、有机酸和脂肪酸方面存在差异。经R-DCPP处理后，叶球微生物多样性降低，群落稳定性增加，而经S-DCPP处理后，叶球微生物群落稳定性降低。相关分析表明，手性除草剂可能通过影响叶片代谢而影响叶圈微生物群落，而糖和萜类化合物被认为是影响叶圈微生物群落结构的主要因素。本研究为通过调节叶片代谢来评价DCPP残留对植物生理及叶圈微生物变化的影响提供了一个新的视角，阐明了DCPP对映体在农业上应用的生态风险。
Dichlorprop (2-(2,4-dichlorophenoxy) propionic acid, DCPP), a commonly used herbicide for weed control, can be residually detected in soil. It is still unclear whether chiral DCPP exerts an enantioselective adverse effect on plant metabolism and the microbial community of the phyllosphere. In this study, we selected Arabidopsis thaliana as a model plant to explore the effects of R- and S-DCPP enantiomers on plant physiological activities, metabolism, and associated changes in the phyllosphere microbial community. Results indicated that the fresh weight of plants decreased by 37.6% after R-DCPP treatment, whereas it increased by 7.6% after S-DCPP treatment. The R-DCPP enantiomer also caused stronger disturbance to leaf morphology, mesophyll cell structure, and leaf metabolites compared with S-DCPP. GC-MS analysis of DCPP-treated Arabidopsis leaves pointed out a differential profile mostly in carbohydrates, organic acids, and fatty acids, between S-DCPP and R-DCPP treatments. The diversity of phyllospheric microorganisms decreased and the stability of microbial community in the phyllosphere increased after R-DCPP treatment, whereas the opposite result was detected after S-DCPP exposure. The correlation analysis revealed that chiral herbicides may affect microbial communities in the phyllosphere by influencing leaf metabolism, while sugars and terpenoids were considered the main factors in reshaping the microbial community structure in the phyllosphere. Our study provides a new perspective for evaluating the effect of residual DCPP enantiomers on plant physiology and corresponding phyllosphere microorganism changes via the regulation of leaf metabolism, and clarifies the ecological risk of DCPP enantiomer application in agriculture.
登录
查看更多内容
手性农药的对映选择性环境毒理学。
影响因子：
4.1
作者：
Ye, Jing;Zhao, Meirong;Niu, Lili;Liu, Weiping
通讯作者：
Liu, Weiping
咪草烟残留物对拟南芥代谢谱和叶际微生物群落的对映选择性影响
影响因子：
6.9
作者：
Zhao Qianqiu;Liu Wanyue;Li Yan;Ke Mingjing;Qu Qian;Yuan Wenting;Pan Xiangliang;Qian Haifeng
通讯作者：
Qian Haifeng
影响因子：
15.5
作者：
Kim, Hyun;Lee, Kiseok Keith;Lee, Yong-Hwan
通讯作者：
Lee, Yong-Hwan
丰富而不是稀有的细菌类群在环境干扰下维持农业土壤微生物组中的主导作用
影响因子：
8.8
作者：
Jiao, Shuo;Wang, Junman;Lu, Yahai
通讯作者：
Lu, Yahai
影响因子：
5.8
作者：
Kembel, Steven W.;Cowan, Peter D.;Webb, Campbell O.
通讯作者：
Webb, Campbell O.