Diverse molecular signatures for ribosomally 'active' Perkinsea in marine sediments.
Diverse molecular signatures for ribosomally 'active' Perkinsea in marine sediments.
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DOI：
10.1186/1471-2180-14-110
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发表时间：
2014-04-29
期刊：
影响因子：
4.2
通讯作者：
Richards TA
中科院分区：
文献类型：
--
作者：
Chambouvet A;Berney C;Romac S;Audic S;Maguire F;De Vargas C;Richards TA
珀金丝虫是真核生物肺泡超门中的一种寄生谱系。最近的研究利用环境小亚单位核糖体RNA基因（SSU rDNA）测序方法，在淡水环境中发现了珀金西样种型的显著多样性和丰富性。相比之下，仅从海洋样品中检索到少量的Perkinsea环境序列，仅培养了两组Perkinsea并进行了形态学描述，它们是海洋软体动物或海洋原生生物的寄生虫。这两个海洋群体形成了独立的、远亲的系统发育集群，由SSU rDNA树上密切相关的谱系组成。在这里，我们检验了一个假设，即perkins是迄今为止在海洋环境中采样不足的群体。利用454多样性“标签”测序技术，研究了这些原生生物在海洋沉积物和水柱样品中的多样性和分布，这些样品取自深叶绿素最大值（DCM）和亚表层，以DNA和RNA为源模板，采样了四个欧洲近海地点。我们检测到265个序列具有已知的Perkinsea分支，其中大部分来自海洋沉积物。此外，这些序列中有27%来自RNA衍生的cDNA文库。系统发育分析将这些序列的大部分分类为38个簇群（包括30个新的海洋簇群），它们的序列相似性低于97%，这表明这种多样性包括一系列生物学和生态学上独特的生物。这些结果表明，Perkinsea谱系比以前在海洋环境中发现的要多样化得多。这种广泛的珀金斯样原生生物多样性主要来自海洋沉积物，其中很大一部分是在RNA衍生文库中检测到的，这表明这种多样性代表了核糖体“活跃”和完整的细胞。鉴于已知的Perkinsea寄生虫感染宿主的系统发育范围，这些数据表明，Perkinsea要么在海洋沉积物中扮演着重要的但迄今为止尚未被认识到的寄生虫角色，要么这一群体的成员可能作为“种子库”微生物群落的一部分存在于海洋沉积物中。
Perkinsea are a parasitic lineage within the eukaryotic superphylum Alveolata. Recent studies making use of environmental small sub-unit ribosomal RNA gene (SSU rDNA) sequencing methodologies have detected a significant diversity and abundance of Perkinsea-like phylotypes in freshwater environments. In contrast only a few Perkinsea environmental sequences have been retrieved from marine samples and only two groups of Perkinsea have been cultured and morphologically described and these are parasites of marine molluscs or marine protists. These two marine groups form separate and distantly related phylogenetic clusters, composed of closely related lineages on SSU rDNA trees. Here, we test the hypothesis that Perkinsea are a hitherto under-sampled group in marine environments. Using 454 diversity ‘tag’ sequencing we investigate the diversity and distribution of these protists in marine sediments and water column samples taken from the Deep Chlorophyll Maximum (DCM) and sub-surface using both DNA and RNA as the source template and sampling four European offshore locations. We detected the presence of 265 sequences branching with known Perkinsea, the majority of them recovered from marine sediments. Moreover, 27% of these sequences were sampled from RNA derived cDNA libraries. Phylogenetic analyses classify a large proportion of these sequences into 38 cluster groups (including 30 novel marine cluster groups), which share less than 97% sequence similarity suggesting this diversity encompasses a range of biologically and ecologically distinct organisms. These results demonstrate that the Perkinsea lineage is considerably more diverse than previously detected in marine environments. This wide diversity of Perkinsea-like protists is largely retrieved in marine sediment with a significant proportion detected in RNA derived libraries suggesting this diversity represents ribosomally ‘active’ and intact cells. Given the phylogenetic range of hosts infected by known Perkinsea parasites, these data suggest that Perkinsea either play a significant but hitherto unrecognized role as parasites in marine sediments and/or members of this group are present in the marine sediment possibly as part of the ‘seed bank’ microbial community.
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影响因子：
4.4
作者：
Lefranc, M;Thénot, A;Debroas, D
通讯作者：
Debroas, D
影响因子：
1.4
作者：
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通讯作者：
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影响因子：
4.4
作者：
Lepere, Cecile;Domaizon, Isabelle;Debroas, Didier
通讯作者：
Debroas, Didier
影响因子：
4.4
作者：
Mangot, Jean-Francois;Lepere, Cecile;Domaizon, Isabelle
通讯作者：
Domaizon, Isabelle
影响因子：
56.9
作者：
Chambouvet, Aurelie;Morin, Pascal;Guillou, Laure
通讯作者：
Guillou, Laure