Expression of blood dendritic cell antigens (BDCAs) by CD1a+ human pulmonary cells.
Expression of blood dendritic cell antigens (BDCAs) by CD1a+ human pulmonary cells.
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CD1a 人肺细胞表达血液树突状细胞抗原 (BDCA)。
DOI：
10.1016/j.rmed.2009.02.006
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发表时间：
2009
影响因子：
4.3
通讯作者：
Tsoumakidou M
中科院分区：
文献类型：
--
作者：
Tsoumakidou M
根据人肺中血DC抗原1-3（BDCAs）的差异表达，最近在人肺中鉴定了髓样和浆细胞样树突状细胞（DC）亚群。方法利用体外细胞培养系统，我们成功地从人肺组织中分离了一群相对纯的（>70%）CD 1a+细胞（n=5个受试者样品）并用针对骨髓DC标志物BDCA 1（CD 1c）和BDCA 3（CD 303）的抗体对这些样品进行染色，浆细胞样DC标记物BDCA 2（CD 141）、朗格罕氏细胞标记物Langerin和成熟标记物CD 83。在不同的受试者样品中，分离的CD 1a+细胞显示Langerin、BDCA和CD 83的可变表达。有趣的是，在两个受试者的样品，其中包含> 70%CD83+成熟的CD 1a+细胞，>50%的细胞是阳性的所有的BDCAs.CONCLUSIONSWe的结论，孤立的肺CD 1a + DC在体外有能力表达髓样和浆细胞样BDCA标记，而不是在肺DC的子集限制，一个显着程度的灵活性/可塑性可以诱导，虽然实验。
BACKGROUNDMyeloid and plasmacytoid dendritic cell (DC) subsets have been recently identified in the human lung based on their differential expression of Blood DC Antigens 1–3 (BDCAs). We investigated the expression of these antigens by isolated human pulmonary CD1a+DCs, namely Langerhan's cells.METHODSUsing an in vitro cell culture system we successfully isolated a population of relatively pure (>70%) CD1a+cells from human lung tissue (n=5 subject samples) and stained these with antibodies against the myeloid DC markers BDCA1 (CD1c) and BDCA3 (CD303), the plasmacytoid DC marker BDCA2 (CD141), the Langerhan's cell marker Langerin and the maturation marker CD83.RESULTSAmong different subject samples, the isolated CD1a+cells showed variable expression of Langerin, BDCAs and CD83. Interestingly, in two subject samples, which contained >70% CD83+mature CD1a+cells, >50% of the cells were positive for all of the BDCAs.CONCLUSIONSWe conclude that isolated pulmonary CD1a+DCs in vitro have the capacity to express both myeloid and plasmacytoid BDCA markers and that rather than subset restriction in pulmonary DCs, a significant degree of flexibility/plasticity can be induced, albeit experimentally.
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