Encapsulation of organic molecules in calcium phosphate nanocomposite particles for intracellular imaging and drug delivery.
Encapsulation of organic molecules in calcium phosphate nanocomposite particles for intracellular imaging and drug delivery.
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DOI：
10.1021/nl8019888
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发表时间：
2008-12
期刊：
影响因子：
10.8
通讯作者：
Adair JH
中科院分区：
文献类型：
--
作者：
Morgan TT;Muddana HS;Altinoglu EI;Rouse SM;Tabaković A;Tabouillot T;Russin TJ;Shanmugavelandy SS;Butler PJ;Eklund PC;Yun JK;Kester M;Adair JH
磷酸钙纳米颗粒（CPNPs）中的成像剂和药物的包封具有作为向细胞和肿瘤递送药物的无毒、生物可吸收载体的潜力。本研究的目的是开发一种用于有机染料和治疗药物的磷酸钙纳米颗粒包封系统，以便可以使用先进的荧光方法来评估药物递送效率和纳米颗粒生物吸收的可能机制。成功合成了包封多种有机荧光团的高浓度CPNP。当与PBS中的游离染料相比时，包封Cy3亚酰胺的良好分散的CPNP表现出荧光量子产率的近5倍增加。FCS扩散数据和细胞染色分别用于显示颗粒的pH依赖性溶解和细胞摄取。此外，一种实验性疏水性细胞生长抑制剂，神经酰胺，成功地在体外通过封装在CPNP中递送到人血管平滑肌细胞。这些研究表明，CPNP是染料和药物的有效载体，用于生物成像，并可能用于治疗干预。
Encapsulation of imaging agents and drugs in calcium phosphate nanoparticles (CPNPs) has potential as a nontoxic, bioresorbable vehicle for drug delivery to cells and tumors. The objectives of this study were to develop a calcium phosphate nanoparticle encapsulation system for organic dyes and therapeutic drugs so that advanced fluoresence methods could be used to assess the efficiency of drug delivery and possible mechanisms of nanoparticle bioabsorption. Highly concentrated CPNPs encapsulating a variety of organic fluorophores were successfully synthesized. Well-dispersed CPNPs encapsulating Cy3 amidite exhibited nearly a 5-fold increase in fluorescence quantum yield when compared to the free dye in PBS. FCS diffusion data and cell staining were used to show pH-dependent dissolution of the particles and cellular uptake, respectively. Furthermore, an experimental hydrophobic cell growth inhibitor, ceramide, was successfully delivered in vitro to human vascular smooth muscle cells via encapsulation in CPNPs. These studies demonstrate that CPNPs are effective carriers of dyes and drugs for bioimaging and, potentially, for therapeutic intervention.
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影响因子：
10.8
作者：
Park, Ji Sun;Han, Tae Hee;Cho, Yong Woo
通讯作者：
Cho, Yong Woo
影响因子：
3.9
作者：
Li, T;Moon, J;Adair, JH
通讯作者：
Adair, JH
DOI：
10.1002/cyto.990110313
发表时间：
1990-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
SOUTHWICK, PL;ERNST, LA;WAGGONER, AS
通讯作者：
WAGGONER, AS
影响因子：
10.8
作者：
Ow, H;Larson, DR;Wiesner, U
通讯作者：
Wiesner, U
影响因子：
8.6
作者：
Sarda, S;Heughebaert, M;Lebugle, A
通讯作者：
Lebugle, A