Microbial diversity and metaproteomic analysis of activated sludge responses to naphthalene and anthracene exposure.
Microbial diversity and metaproteomic analysis of activated sludge responses to naphthalene and anthracene exposure.
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活性污泥对萘和蒽暴露反应的微生物多样性和宏蛋白质组学分析
作者：
活性污泥法可以通过生物降解有效去除废水中的多环芳烃。然而，参与这一过程的可降解微生物和功能酶仍不清楚。在这项研究中，我们成功地采用了一个实验室规模的序批式反应器，调查微生物群落和蛋白质表达的变化，响应于添加不同的多环芳烃和处理时间。通过16 S rRNA基因454焦磷酸测序分析细菌群落结构表明，经多环芳烃处理的污泥中以伯克氏菌目细菌为主。所有污泥样品的2D蛋白质谱进行的质谱分析表明，在该过程中表现出差异表达谱的大多数蛋白质来自伯克氏菌群，这些蛋白质参与DNA复制，脂肪酸和葡萄糖代谢，应激反应，蛋白质合成和芳烃代谢。然而，蛋白质表达谱表明，萘，而不是蒽，可以诱导多环芳烃降解蛋白的表达，并加速其从污泥中的消除。虽然实验组中只添加了萘和蒽，但也检测到了参与其他多环芳烃（尤其是联苯）代谢的差异表达酶。这项研究提供了明显的证据连接伯克霍尔德菌种群的代谢活动与多环芳烃的降解活性污泥法。本研究结果表明，高通量测序技术结合微生物多样性分析的元蛋白质组学在环境样品分析中的成功应用，为监测蛋白质表达谱的变化及其与微生物多样性的相关性提供了便利。
The activated sludge process can effectively remove polycyclic aromatic hydrocarbons (PAHs) from wastewater via biodegradation. However, the degradable microorganisms and functional enzymes involved in this process remain unclear. In this study, we successfully employed a laboratory-scale sequential batch reactor to investigate variations in microbial community and protein expression in response to the addition of different PAHs and process time. The analysis of bacterial community structure by 454 pyrosequencing of the 16S rRNA gene indicated that bacteria from Burkholderiales order were dominant in PAHs treated sludge. Mass spectrometry performed with 2D protein profiles of all sludge samples demonstrated that most proteins exhibiting differential expression profiles during the process were derived from Burkholderiales populations; these proteins are involved in DNA replication, fatty acid and glucose metabolism, stress response, protein synthesis, and aromatic hydrocarbon metabolism. Nevertheless, the protein expression profiles indicated that naphthalene, but not anthracene, can induce the expression of PAH-degrading proteins and accelerate its elimination from sludge. Though only naphthalene and anthracene were added into our experimental groups, the differentially expressed enzymes involved in other PAHs (especially biphenyl) metabolism were also detected. This study provides apparent evidence linking the metabolic activities of Burkholderiales populations with the degradation of PAHs in activated sludge processes. Overall, our findings highlighted the successful application of metaproteomics integrated with microbial diversity analysis by high-throughput sequencing technique on the analysis of environmental samples, which could provide a convenience to monitor the changes in proteins expression profiles and their correlation with microbial diversity.
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DOI：
10.1007/bf01403354
发表时间：
1969-01-01
期刊：
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG
影响因子：
--
作者：
EISENBRAND, J;BAUMANN, K
通讯作者：
BAUMANN, K
影响因子：
3.6
作者：
Maron, Pierre-Alain;Ranjard, Lionel;Lemanceau, Philippe
通讯作者：
Lemanceau, Philippe
影响因子：
11
作者：
Benndorf, Dirk;Balcke, Gerd U.;von Bergen, Martin
通讯作者：
von Bergen, Martin
影响因子：
3.6
作者：
Hesketh, AR;Chandra, G;Chater, KF
通讯作者：
Chater, KF
影响因子：
1.8
作者：
Knightes, CD;Peters, CA
通讯作者：
Peters, CA