Role of hepatic lipase in intermediate-density lipoprotein and small, dense low-density lipoprotein formation in hemodialysis patients.
Role of hepatic lipase in intermediate-density lipoprotein and small, dense low-density lipoprotein formation in hemodialysis patients.
复制标题
肝脂肪酶在血液透析患者中密度脂蛋白和小而密的低密度脂蛋白形成中的作用。
DOI：
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复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
T. Hosoya
中科院分区：
文献类型：
--
作者：
K. Oi;T. Hirano;S. Sakai;Y. Kawaguchi;T. Hosoya
背景
据报道，残余脂蛋白和小而密的低密度脂蛋白（LDL）是心血管疾病的危险因素。为了确定这些危险因素是否存在于动脉粥样硬化血管疾病高发的血液透析（HD）患者中，我们测量了HD患者的残余脂蛋白浓度和LDL颗粒直径，并与对照组进行比较。我们还测量了脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（HTGL），在残余脂蛋白和小而致密的LDL的产生中起重要作用，我们将这些变化与HD患者的血浆脂蛋白异常相关联。
方法
超离心分离脂蛋白。采用酶联免疫吸附法测定极低密度脂蛋白（VLDL）和中密度脂蛋白（IDL）组分中的载脂蛋白B。通过梯度凝胶电泳测量LDL颗粒的平均直径。
结果
血液透析患者和对照组的血浆甘油三酯、总胆固醇和高密度脂蛋白（HDL）胆固醇浓度相当，而血液透析患者的LDL胆固醇显著降低。平均LDL颗粒直径在HD患者和对照组之间没有显著差异。LDL颗粒直径与所有受试者的血浆甘油三酯浓度呈负相关。HD患者和对照组的VLDL甘油三酯、VLDL胆固醇和VLDL载脂蛋白B相当。与对照组相比，HD患者的IDL甘油三酯、IDL胆固醇和IDL载脂蛋白B浓度均显著升高。HD患者LPL质量没有改变，但HTGL活性显著降低。HTGL活性与IDL浓度呈负相关。
结论
这些结果表明，HD患者脂蛋白异常的一个突出特点是IDL颗粒数量显着增加。此外，小而密的LDL与尿毒症血脂异常无关。由于HTGL促进IDL向LDL的转化和贫脂LDL的产生，HTGL活性的降低可能有助于IDL颗粒的积累，并可能阻止HD患者中小而致密的LDL形成。
BACKGROUND
It has been reported that remnant lipoproteins and small, dense low-density lipoproteins (LDLs) are risk factors for cardiovascular disease. To determine whether these risk factors are present in hemodialysis (HD) patients who are suffering from a high incidence of atherosclerotic vascular disease, we measured concentrations of remnant lipoproteins and LDL particle diameter in HD patients and compared these with controls. We also measured lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) that play important roles in the generation of remnant lipoproteins and small, dense LDL, and we correlated these changes with plasma lipoprotein abnormalities in HD patients.
METHODS
Lipoproteins were separated by ultracentrifugation. Apoprotein B in very low-density lipoprotein (VLDL), and intermediate-density lipoprotein (IDL) fractions were measured by a sensitive enzyme-linked immunosorbent assay method. The average LDL particle diameter was measured by gradient gel electrophoresis.
RESULTS
Plasma triglyceride, total cholesterol, and high-density lipoprotein (HDL) cholesterol concentrations were comparable between HD patients and controls, whereas LDL cholesterol was significantly lower in HD patients. The average LDL particle diameter was not significantly different between HD patients and controls. LDL particle diameter was inversely related to plasma triglyceride concentrations in all of the subjects. VLDL triglyceride, VLDL cholesterol, and VLDL apoprotein B were comparable between HD patients and controls. IDL triglyceride, IDL cholesterol, and IDL apoprotein B concentrations were all significantly increased in HD patients compared with those in controls. LPL mass was not altered, but HTGL activity was significantly decreased in HD patients. The HTGL activity was inversely related to IDL concentrations.
CONCLUSIONS
These results suggest that a prominent characteristic of lipoprotein abnormalities in HD patients is a marked increase in IDL particle number. In addition, small, dense LDL is not associated with uremic dyslipidemia. Because HTGLs promote the conversion from IDL to LDL and the generation of lipid-poor LDL, a decrease in HTGL activity may contribute to the accumulation of IDL particle and may prevent small, dense LDL formation in HD patients.
DOI：
10.1001/jama.1988.03410130125037
发表时间：
1988-10
期刊：
JAMA
影响因子：
--
作者：
M. Austin;J. Breslow;C. Hennekens;Julie E. Buring;W. Willett;R. Krauss
通讯作者：
M. Austin;J. Breslow;C. Hennekens;Julie E. Buring;W. Willett;R. Krauss
影响因子：
158.5
作者：
Herzog, CA;Ma, JZ;Collins, AJ
通讯作者：
Collins, AJ