Distribution and Diversity of Comammox Nitrospira in Coastal Wetlands of China.
Distribution and Diversity of Comammox Nitrospira in Coastal Wetlands of China.
复制标题
中国滨海湿地comammox Nitrospira的分布及多样性
DOI：
10.3389/fmicb.2020.589268
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Han P
中科院分区：
文献类型：
--
作者：
Sun D;Tang X;Zhao M;Zhang Z;Hou L;Liu M;Wang B;Klümper U;Han P
完全氨氧化剂（comammox）能够单独将氨氧化为硝酸盐，被认为在全球氮循环中发挥重要作用。然而，该菌在河口潮滩湿地的分布及影响其丰度和多样性的环境驱动因素尚不清楚。本文对我国河口滩涂湿地沿着的硝化螺菌的地理分布进行了大规模调查，在16个采样点中的9个点成功检出了硝化螺菌。硝化螺旋菌的丰度范围为5.19 × 103 ~ 8.33 × 104拷贝/g，比氨氧化细菌（AOB）和氨氧化古菌（AOA）低2.21 ~ 5.44倍。基于氨单加氧酶编码基因（amoA）α亚基的系统发育分析表明，主要来源于上游河流输入的Comammox Nitrospira Clade A占所检测到的Comammox Nitrospira的80%以上，而Comammox Nitrospira Clade B很少被检测到。在河口样品中，Comammox Nitrospira丰度和占优势的Comammox Nitrospira OTUs各不相同，显示出地理格局。盐度和pH值是影响硝化螺菌在河口潮滩湿地分布的重要环境因子。comammox Nitrospira的丰度进一步与高氨和亚硝酸盐浓度呈负相关。总之，这项研究揭示了存在，丰富和分布的comammox硝化螺菌和河口生态系统中的驱动环境因素，从而提供了深入了解这个最近发现的硝化财团的生态位及其在全球河口环境中的硝化作用的贡献。
Complete ammonia oxidizers (comammox), able to individually oxidize ammonia to nitrate, are considered to play a significant role in the global nitrogen cycle. However, the distribution of comammox Nitrospira in estuarine tidal flat wetland and the environmental drivers affecting their abundance and diversity remain unknown. Here, we present a large-scale investigation on the geographical distribution of comammox Nitrospira along the estuarine tidal flat wetlands of China, where comammox Nitrospira were successfully detected in 9 of the 16 sampling sites. The abundance of comammox Nitrospira ranged from 5.19 × 103 to 8.33 × 104 copies/g, 2.21 to 5.44-folds lower than canonical ammonia oxidizers: ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). Phylogenetic analysis based on the alpha subunit of the ammonia monooxygenase encoding gene (amoA) revealed that comammox Nitrospira Clade A, mainly originating from upstream river inputs, accounts for more than 80% of the detected comammox Nitrospira, whereas comammox Nitrospira clade B were rarely detected. Comammox Nitrospira abundance and dominant comammox Nitrospira OTUs varied within the estuarine samples, showing a geographical pattern. Salinity and pH were the most important environmental drivers affecting the distribution of comammox Nitrospira in estuarine tidal flat wetlands. The abundance of comammox Nitrospira was further negatively correlated with high ammonia and nitrite concentrations. Altogether, this study revealed the existence, abundance and distribution of comammox Nitrospira and the driving environmental factors in estuarine ecosystems, thus providing insights into the ecological niches of this recently discovered nitrifying consortium and their contributions to nitrification in global estuarine environments.
登录
查看更多内容
影响因子：
3.3
作者：
Bernhard, Anne E.;Chelsky, Ariella;Roberts, Brian J.
通讯作者：
Roberts, Brian J.
磺胺二甲嘧啶对河口和沿海沉积物反硝化及相关 N2O 释放的影响
影响因子：
11.4
作者：
Hou, Lijun;Yin, Guoyu;Tong, Chunfu
通讯作者：
Tong, Chunfu
影响因子：
2.1
作者：
Kurola, J;Salkinoja-Salonen, M;Romantschuk, M
通讯作者：
Romantschuk, M
中国东南沿海湿地互花米草入侵时序中的土壤异化硝酸盐还原过程：动力学和环境影响
影响因子：
4.9
作者：
Gao, Dengzhou;Li, Xiaofei;Chen, Xing
通讯作者：
Chen, Xing
影响因子：
3.7
作者：
Lin, Xianbiao;Liu, Min;Gao, Juan
通讯作者：
Gao, Juan