Fine structure of the vaccinia virion determined by controlled degradation and immunolocalization.
Fine structure of the vaccinia virion determined by controlled degradation and immunolocalization.
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DOI：
10.1016/j.virol.2014.11.020
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发表时间：
2015-01-15
期刊：
影响因子：
3.7
通讯作者：
Condit RC
中科院分区：
文献类型：
--
作者：
Moussatche N;Condit RC
The vaccinia virion is a membraned, slightly flattened, barrel-shaped particle, with a complex internal structure featuring a biconcave core flanked by lateral bodies. Although the architecture of the purified mature virion has been intensely characterized by electron microscopy, the distribution of the proteins within the virion has been examined primarily using biochemical procedures. Thus, it has been shown that non-ionic and ionic detergents combined or not with a sulfhydryl reagent can be used to disrupt virions and, to a limited degree, separate the constituent proteins in different fractions. Applying a controlled degradation technique to virions adsorbed on EM grids, we were able to immuno-localize viral proteins within the virion particle. Our results show after NP40 and DTT treatment, membrane proteins are removed from the virion surface revealing proteins that are associated with the lateral bodies and the outer layer of the core wall. Combined treatment using high salt and high DTT removed lateral body proteins and exposed proteins of the internal core wall. Cores treated with proteases could be disrupted and the internal components were exposed. Cts8, a mutant in the A3 protein, produces aberrant virus that, when treated with NP-40 and DTT, release to the exterior the virus DNA associated with other internal core proteins. With these results, we are able to propose a model for the structure the vaccinia virion.
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影响因子：
7.8
作者：
DALES, S
通讯作者：
DALES, S
细胞内成熟疫苗病毒的结构和组装： 分离粒子分析
影响因子：
5.4
作者：
Griffiths, G;Wepf, R;Roos, N
通讯作者：
Roos, N
影响因子：
5.4
作者：
DUBOCHET, J;ADRIAN, M;WITTEK, R
通讯作者：
WITTEK, R
影响因子：
2.7
作者：
G贸mez, CE;Esteban, M
通讯作者：
Esteban, M
DOI：
10.1083/jcb.10.4.475
发表时间：
1961-08
期刊：
The Journal of biophysical and biochemical cytology
影响因子：
--
作者：
DALES, S;SIMINOVITCH, L
通讯作者：
SIMINOVITCH, L