Distribution of anthocyanins delivered from a bioadhesive black raspberry gel following topical intraoral application in normal healthy volunteers.
Distribution of anthocyanins delivered from a bioadhesive black raspberry gel following topical intraoral application in normal healthy volunteers.
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DOI：
10.1007/s11095-008-9806-x
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发表时间：
2009-04
影响因子：
3.7
通讯作者：
Mallery, Susan R.
中科院分区：
文献类型：
--
作者：
Ugalde, Carlos M.;Liu, Zhongfa;Ren, Chen;Chan, Kenneth K.;Rodrigo, Kapila A.;Ling, Yhonghua;Larsen, Peter E.;Chacon, Guillermo E.;Stoner, Gary D.;Mumper, Russell J.;Fields, Henry W.;Mallery, Susan R.
我们的口腔化学预防试验的结果表明，关于冻干黑树莓（FBR）凝胶的化学预防功效，患者间存在明显差异。我们推测这些数据反映了关键FBR化合物（花青素）的吸收、靶组织摄取和局部化合物代谢的个体患者相关差异。因此，本研究评估了来自10%（w/w）FBR凝胶的花青素在唾液、口腔组织和血浆中的分布。人类受试者的参与需要收集：（1）唾液、组织和血浆（凝胶施用后5分钟，角化组织），（2）唾液和血浆（舌下凝胶施用后5分钟），（3）凝胶施用后1、2和4小时的唾液和血浆（角化组织），和（4）唾液（矢车菊素3-芸香糖苷孵育物）。通过LC/MS/MS分析相应样品中的FBR花青素的水平。我们的数据显示：相对于匹配的血浆样品，唾液和口腔组织中的花青素水平显著更高，花青素摄取的显著供体特异性变化，花青素在靶位点的可持续性，pH影响花青素渗透和口内花青素分解和/或代谢。以前没有口腔化学预防试验评价化合物在治疗部位的分布。我们的数据，这表明了当地交付衍生的药理学优势，提供了见解，可以推进口腔化学预防策略。
Results from our oral cavity chemoprevention trial demonstrated appreciable interpatient variations regarding chemopreventive efficacy of a freeze dried black raspberry (FBR) gel. We speculated these data reflected individual patient-related differences in absorption, target tissue uptake and local compound metabolism of key FBR compounds (anthocyanins). Accordingly, this study assessed the distribution of anthocyanins from the 10% (w/w) FBR gel in saliva, oral tissues and plasma. Human subject participation entailed collection of: (1) saliva, tissue and plasma (5 min following gel application, keratinized tissues), (2) saliva and plasma (5 min after sublingual gel application), (3) saliva and plasma at 1, 2, and 4 h post gel application (keratinized tissues), and (4) saliva (cyanidin 3-rutinoside incubations). Levels of FBR anthocyanins in the respective samples were analyzed by LC/MS/MS. Our data show: significantly higher anthocyanin levels in saliva and oral tissues relative to matched plasma samples, marked donor-specific variations in anthocyanin uptake, sustainability of anthocyanins at the target site, pH affects anthocyanin penetration and intraoral anthocyanin decomposition and/or metabolism. No previous oral cavity chemoprevention trials evaluated compound distribution at the treatment site. Our data, which demonstrate a local delivery-derived pharmacologic advantage, provide insights which could advance oral cavity chemoprevention strategies.
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影响因子：
2.9
作者：
Rodrigo, Kapila A.;Rawal, Yeshwant;Mallery, Susan R.
通讯作者：
Mallery, Susan R.
影响因子：
11.5
作者：
Lippman, SM;Lee, JJ;Lotan, R
通讯作者：
Lotan, R
影响因子：
3.7
作者：
Mallery, Susan R.;Stoner, Gary D.;Mumper, Russell J.
通讯作者：
Mumper, Russell J.
影响因子：
45.3
作者：
Rudin, CM;Cohen, EEW;Vokes, EE
通讯作者：
Vokes, EE
影响因子：
11.5
作者：
Mulshine, JL;Atkinson, JC;Cavanaugh, PF
通讯作者：
Cavanaugh, PF