Polyethyleneimine functionalized polymer microsphere: a novel delivery vector for cells
Polyethyleneimine functionalized polymer microsphere: a novel delivery vector for cells
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聚乙烯亚胺功能化聚合物微球：一种新型细胞递送载体
DOI：
10.1039/c2py20012k
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发表时间：
2012-04
影响因子：
4.6
通讯作者：
Feng, Xizeng
中科院分区：
文献类型：
--
作者：
Gao, Chunying;Zhang, Han;Wu, Ming;Liu, Yang;Wu, Yipan;Yang, Xinlin;Feng, Xizeng
在纯乙腈中通过精馏沉淀法合成了直径分别为69 nm和160 nm的单分散聚乙二醇二甲基丙烯酸-共甲基丙烯酸（P(EGDMA-co-MAA)）微球，这些微球通过氢键和静电相互作用与聚乙烯亚胺（PEI）链上的氨基相互作用形成PEI功能化（P(EGDMA-co-MAA)）。动态光散射（DLS）和琼脂糖凝胶电泳证实了PEI修饰P（EGDMA-co-MAA）微球的成功。采用人胚胎肾（HEK） 293T细胞与P（EGDMA-co-MAA）微球/质粒DNA （pDNA）复合物共培养的方法评价体外基因转染效果，并采用荧光显微镜观察结果。此外，通过MTT法和FDA-PI染色评估pei功能化P（EGDMA-co-MAA）微球的细胞毒性。pei功能化的(P（EGDMA-co-MAA）提供了一种潜在的新型细胞递送载体。
Monodisperse poly(ethylene glycol dimethacrylate-co-methacrylic acid) (P(EGDMA-co-MAA)) microspheres with two different diameters (69 and 160 nm) were facilely synthesized via distillation precipitation polymerization in neat acetonitrile, and these microspheres interacted with the amino groups of polyethyleneimine (PEI) chains though hydrogen-bonding as well as electrostatic interactions to form PEI-functionalized (P(EGDMA-co-MAA)). The successful modification of P(EGDMA-co-MAA) microspheres with PEI was confirmed by dynamic light scattering (DLS) and agarose gel electrophoresis. The in vitro gene transfection was evaluated by co-culturing human embryonic kidney (HEK) 293T cells and P(EGDMA-co-MAA) microsphere/plasmid DNA (pDNA) complexes, and the results were determined by fluorescence microscopy. Furthermore, the cytotoxicity of PEI-functionalized P(EGDMA-co-MAA) microspheres was assessed via MTT assay and FDA–PI staining. The PEI-functionalized (P(EGDMA-co-MAA) provides a potential novel delivery vector for cells.
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影响因子：
2.9
作者：
S. Hou;Kun Yang;Xizeng Feng
通讯作者：
S. Hou;Kun Yang;Xizeng Feng
影响因子：
10.8
作者：
Nasongkla, Norased;Bey, Erik;Gao, Jinming
通讯作者：
Gao, Jinming
影响因子：
6.2
作者：
Simone, Eric A.;Dziubla, Thomas D.;Muzykantov, Vladimir R.
通讯作者：
Muzykantov, Vladimir R.
DOI：
10.1016/j.colsurfa.2008.03.006
发表时间：
2008-06-05
影响因子：
5.2
作者：
Li, Guoliang;Yang, Xinlin;Wang, Junyou
通讯作者：
Wang, Junyou
影响因子：
29.4
作者：
Leyang Zhang;Rui Guo;Mi Yang;Xiqun Jiang;Baorui Liu
通讯作者：
Leyang Zhang;Rui Guo;Mi Yang;Xiqun Jiang;Baorui Liu