Role of mutation in Pseudomonas aeruginosa biofilm development.
Role of mutation in Pseudomonas aeruginosa biofilm development.
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DOI：
10.1371/journal.pone.0006289
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发表时间：
2009-07-16
期刊：
影响因子：
3.7
通讯作者：
Webb JS
中科院分区：
文献类型：
--
作者：
Conibear TC;Collins SL;Webb JS
细菌在自然界中的生存能力大大增强了它们在称为生物膜的表面相关群落中生长的能力。通常，生物膜产生细菌细胞的增殖，称为小菌落，其是细菌生长的高度不稳定的三维焦点。微菌落生长仅由生物膜内的细菌亚群启动，但对这种分化的过程仍知之甚少。在生物膜内细菌之间的拥挤和激烈竞争的条件下，微进化过程如突变选择可能对生长很重要;然而，它们对基于微菌落的生物膜生长和结构的影响以前没有被探索过。为了研究生物膜内的原位突变，我们用含有+1移码突变的绿色荧光蛋白基因转化铜绿假单胞菌细胞。转化的铜绿假单胞菌细胞是无荧光的，直到发生导致回复到野生型序列的突变。在微菌落结构中观察到了诱导突变，但在其他生物膜细胞或铜绿假单胞菌细胞的增殖培养物中未观察到。因此，小菌落可能代表生物膜内突变和进化的重要焦点。我们计算出微菌落特异性突变频率的增加至少是100倍，与无菌生长的文化。我们还观察到，增变基因表型可以增强铜绿假单胞菌细胞的基于微菌落的生长。对于DNA保真度和错误修复缺陷的铜绿假单胞菌菌株，我们发现随着生物体突变频率的增加，小菌落的起始和生长得到增强。我们认为，基于微菌落的生长可能涉及突变和随后选择的突变体更好地适应在拥挤的细胞环境中的表面上生长。这种生物膜生长模型类似于肿瘤进展和肿瘤发展期间发生的突变选择，并且可能有助于解释为什么结构和遗传异质性是细菌生物膜群体的特征。
The survival of bacteria in nature is greatly enhanced by their ability to grow within surface-associated communities called biofilms. Commonly, biofilms generate proliferations of bacterial cells, called microcolonies, which are highly recalcitrant, 3-dimensional foci of bacterial growth. Microcolony growth is initiated by only a subpopulation of bacteria within biofilms, but processes responsible for this differentiation remain poorly understood. Under conditions of crowding and intense competition between bacteria within biofilms, microevolutionary processes such as mutation selection may be important for growth; however their influence on microcolony-based biofilm growth and architecture have not previously been explored. To study mutation in-situ within biofilms, we transformed Pseudomonas aeruginosa cells with a green fluorescent protein gene containing a +1 frameshift mutation. Transformed P. aeruginosa cells were non-fluorescent until a mutation causing reversion to the wildtype sequence occurs. Fluorescence-inducing mutations were observed in microcolony structures, but not in other biofilm cells, or in planktonic cultures of P. aeruginosa cells. Thus microcolonies may represent important foci for mutation and evolution within biofilms. We calculated that microcolony-specific increases in mutation frequency were at least 100-fold compared with planktonically grown cultures. We also observed that mutator phenotypes can enhance microcolony-based growth of P. aeruginosa cells. For P. aeruginosa strains defective in DNA fidelity and error repair, we found that microcolony initiation and growth was enhanced with increased mutation frequency of the organism. We suggest that microcolony-based growth can involve mutation and subsequent selection of mutants better adapted to grow on surfaces within crowded-cell environments. This model for biofilm growth is analogous to mutation selection that occurs during neoplastic progression and tumor development, and may help to explain why structural and genetic heterogeneity are characteristic features of bacterial biofilm populations.
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影响因子：
7
作者：
Lewenza, S;Falsafi, RK;Hancock, REW
通讯作者：
Hancock, REW
DOI：
10.1073/pnas.0607057103
发表时间：
2006-11-28
影响因子：
11.1
作者：
Bielas, Jason H.;Loeb, Keith R.;Loeb, Lawrence A.
通讯作者：
Loeb, Lawrence A.
影响因子：
3
作者：
Häussler, S;Ziegler, I;Steinmetz, I
通讯作者：
Steinmetz, I
影响因子：
3.2
作者：
Allegrucci, Magee;Sauer, Karin
通讯作者：
Sauer, Karin
DOI：
10.1016/s1383-5718(98)00043-6
发表时间：
1998-05-11
影响因子：
1.9
作者：
Cariello, NF;Narayanan, S;Casey, WM
通讯作者：
Casey, WM