Icariside II Restores Vascular Smooth Muscle Cell Contractile Phenotype by Enhancing the Focal Adhesion Signaling Pathway in the Rat Vascular Remodeling Model.
Icariside II Restores Vascular Smooth Muscle Cell Contractile Phenotype by Enhancing the Focal Adhesion Signaling Pathway in the Rat Vascular Remodeling Model.
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Icariside II 通过增强大鼠血管重塑模型中的局灶粘附信号通路恢复血管平滑肌细胞收缩表型
DOI：
10.3389/fphar.2022.897615
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发表时间：
2022
影响因子：
5.6
通讯作者：
Wen, Hao
中科院分区：
文献类型：
--
作者：
Lv, Junyuan;Li, Xintong;Wu, Hongyu;Li, Jiayang;Luan, Boyang;Li, Yiqi;Li, Yeli;Yang, Danli;Wen, Hao
Vascular smooth muscle cell (VSMC) phenotypic transition represents the fundamental pathophysiological alteration in the vascular remodeling process during the initiation and progression of cardiovascular diseases. Recent studies have revealed that Icariside II (ICS-II), a flavonol glycoside derived from the traditional Chinese medicine Herba Epimedii, exhibited therapeutic effects in various cardiovascular diseases. However, the therapeutic efficacy and underlying mechanisms of ICS-II regarding VSMC phenotypic transition were unknown. In this study, we investigated the therapeutic effects of ICS-Ⅱ on vascular remodeling with a rat’s balloon injury modelin vivo. The label-free proteomic analysis was further implemented to identify the differentially expressed proteins (DEPs) after ICS-II intervention. Gene ontology and the pathway enrichment analysis were performed based on DEPs. Moreover, platelet-derived growth factor (PDGF-BB)-induced primary rat VSMC was implemented to verify the restoration effects of ICS-II on the VSMC contractile phenotype. Results showed that ICS-II could effectively attenuate the vascular remodeling process, promote SMA-α protein expression, and inhibit OPN expressionin vivo. The proteomic analysis identified 145 differentially expressed proteins after ICS-II intervention. Further, the bioinformatics analysis indicated that the focal adhesion signaling pathway was enriched in the ICS-II group.In vitrostudies showed that ICS-II suppressed VSMC proliferation and migration, and promoted VSMC contractile phenotype by modulating the focal adhesion signaling pathway. Taken together, our results suggest that ICS-II attenuates the vascular remodeling process and restores the VSMC contractile phenotype by promoting the focal adhesion pathway.
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影响因子：
8.3
作者：
Canes, Laia;Alonso, Judith;Martinez-Gonzalez, Jose
通讯作者：
Martinez-Gonzalez, Jose
DOI：
10.1042/cs20201528
发表时间：
2021-05-14
期刊：
Clinical science (London, England : 1979)
影响因子：
--
作者：
Ribeiro-Silva, J C;Miyakawa, A A;Krieger, Jose E
通讯作者：
Krieger, Jose E
影响因子：
7.9
作者：
Al-Shehabi, Tuqa Saleh;Iratni, Rabah;Eid, Ali H.
通讯作者：
Eid, Ali H.
影响因子：
8.7
作者：
Kiechl, S;Willeit, J
通讯作者：
Willeit, J
VEGF-A 通过心肌素的亚硝基化刺激 STAT3 活性，调节血管平滑肌细胞分化标志物的表达
影响因子：
4.6
作者：
Liao XH;Xiang Y;Li H;Zheng L;Xu Y;Xi Yu C;Li JP;Zhang XY;Xing WB;Cao DS;Bao LY;Zhang TC
通讯作者：
Zhang TC