Site-specific expression of mRNAs for osteonectin, osteocalcin, and osteopontin revealed by in situ hybridization in rat periodontal ligament during physiological tooth movement.
Site-specific expression of mRNAs for osteonectin, osteocalcin, and osteopontin revealed by in situ hybridization in rat periodontal ligament during physiological tooth movement.
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通过原位杂交揭示了生理性牙齿移动过程中大鼠牙周膜中骨连接蛋白、骨钙蛋白和骨桥蛋白 mRNA 的位点特异性表达。
DOI：
--
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发表时间：
1994
影响因子：
3.2
通讯作者：
S. Nomura
中科院分区：
文献类型：
--
作者：
Teruko TAKANO;T. Takemura;Yukihiko Kitamura;S. Nomura
用含骨钙素(OSC)、骨连蛋白(OSN)和骨桥蛋白(OPN)的非放射性同位素探针，用原位杂交组织化学方法研究了大鼠上颌牙周膜(PDL)中骨钙素(OSC)、骨桥蛋白(OSN)和骨桥蛋白(OPN)的表达。在7周龄SD雄性大鼠上第二磨牙颊根神经根的神经根中，OSN和OSC的mRNAs均呈强阳性表达。成骨细胞和成骨前体细胞表达OSN和OSC mRNAs。OPN mRNA阳性信号分布于颊根根间隔近中面，在生理性牙齿移动过程中，生理性骨吸收受到特异性限制。OPN mRNA表达于骨吸收表面的细胞，包括破骨细胞和骨细胞。OSN mRNA的中等强度阳性信号分布于整个韧带的成纤维细胞中。成牙本质细胞和早熟成牙本质细胞表达OSN和OSC mRNA。成牙骨质细胞和牙骨质细胞表达OSN、OSC和OPN基因。这些发现提示OSC、OSN和OPN在骨重塑、PDL重塑、牙本质形成和牙骨质形成中的生理作用。
We investigated the gene expression for non-collagenous proteins in periodontal ligament (PDL) by in situ hybridization histochemistry with a non-radioisotopic probe with cRNAs for osteocalcin (Osc), osteonectin (Osn), and osteopontin (Opn) in rat maxillary dento-alveolar unit containing molars and intact PDL. A highly intense positive signal for Osn and Osc mRNAs was expressed at all distal surfaces of the interradicular septum of buccal roots of the upper second molar in 7-week-old Sprague-Dawley male rats. Cells showing positive signals for Osn and Osc mRNAs were osteoblasts and osteoprogenitor cells. The distribution of Opn mRNA-positive signal was demonstrable at the mesial surface of the interradicular septum of buccal roots, where physiological bone resorption was specifically restricted during physiological tooth movement. Opn mRNA was expressed in cells on the bone resorption surface, including osteoclasts, and osteocytes. A moderately intense positive signal for Osn mRNA was distributed in fibroblasts throughout the ligament. Odontoblasts and pre-mature odontoblasts exhibited a strong signal for Osn and Osc mRNA. Cementoblasts and cementocytes were positive for Osn, Osc, and Opn mRNAs. These findings suggest physiological roles of Osc, Osn, and Opn in bone remodeling, PDL remodeling, dentinogenesis, and cementogenesis.
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分离培养物中产生牙骨质蛋白的人类肿瘤细胞。
DOI：
10.1016/0169-6009(92)90796-g
发表时间：
1992
期刊：
Bone and mineral
影响因子：
--
作者：
Arzate,H;Olson,SW;Page,RC;Narayanan,AS
通讯作者：
Narayanan,AS
影响因子：
--
作者：
ROBERTS, WE;MOZSARY, PG;KLINGLER, E
通讯作者：
KLINGLER, E
影响因子：
2.9
作者：
ROMBERG, RW;WERNESS, PG;MANN, KG
通讯作者：
MANN, KG
牙骨质形成过程中附着蛋白的表达。
DOI：
--
发表时间：
1990
期刊：
Journal de biologie buccale
影响因子：
--
作者：
Somerman,MJ;Shroff,B;Agraves,WS;Morrison,G;Craig,AM;Denhardt,DT;Foster,RA;Sauk,JJ
通讯作者：
Sauk,JJ
影响因子：
3.2
作者：
MARK, MP;PRINCE, CW;BUTLER, WT
通讯作者：
BUTLER, WT