In vitro evaluation of dentinal tubule penetration and biomineralization ability of a new root-end filling material.

In vitro evaluation of dentinal tubule penetration and biomineralization ability of a new root-end filling material.
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DOI:
10.1016/j.joen.2012.04.017
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发表时间:
2012-08
影响因子:
4.2
通讯作者:
Spears R
Spears R
中科院分区:
医学2区
文献类型:
--
作者:
Bird DC;Komabayashi T;Guo L;Opperman LA;Spears R

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Capasio正在开发成为新一代牙髓材料,可能用作根端填充材料。本研究的目的是比较Capasio和矿物三氧化物聚合物(MTA)穿透人牙本质小管的能力,并检查Capasio和MTA与合成组织液(STF)和根管壁的相互作用。用Capasio或MTA填充根端制备物,使其在STF中凝固4周,然后在离切除表面1、2和3 mm处切片。通过使用扫描电子显微镜(SEM)评价渗透深度。接下来,将Capasio和MTA样品制备为1-g丸粒和根端制备物。将样品置于STF中,使其凝固,然后通过使用SEM、能量色散X射线分析(EDXA)和X射线衍射(XRD)技术表征。在所有水平均观察到Capasio渗透到牙本质小管中。在任何水平均未观察到MTA渗透到牙本质小管中。Capasio和MTA都在上清液中、在它们的暴露表面上以及在界面层中形成磷灰石晶体,当通过使用SEM和EDXA评估时,界面层在结构和元素组成上相似。这些晶体的XRD分析对应于羟基磷灰石的那些报道。当用作根端填充材料时,Capasio更容易渗透牙本质小管。当暴露于STF时,Capasio和MTA都促进磷灰石沉积。
Capasio is being developed as a new generation of endodontic material with potential use as a root-end filling material. The aim of this study was to compare the ability of Capasio and mineral trioxide aggregate (MTA) to penetrate human dentinal tubules and examine the interaction of Capasio and MTA with a synthetic tissue fluid (STF) and root canal walls in extracted human teeth. Root-end preparations were filled with Capasio or MTA, allowed to set for 4 weeks in STF, and then sectioned at 1, 2, and 3 mm from resected surface. Depth of penetration was evaluated by using scanning electron microscopy (SEM). Next, Capasio and MTA samples were prepared both in 1-g pellets and in root-end preparations. Samples were placed in STF, allowed to set, and then characterized by using SEM, energy dispersive x-ray analysis (EDXA), and x-ray diffraction (XRD) techniques. Penetration of Capasio into dentinal tubules was observed at all levels. No penetration of MTA into dentinal tubules was observed at any level. Both Capasio and MTA formed apatite crystals in the supernatant, on their exposed surfaces, and in the interfacial layers that were similar in structure and elemental composition when evaluated by using SEM and EDXA. XRD analysis of these crystals corresponds with those reported for hydroxyapatite. When used as a root-end filling material, Capasio is more likely to penetrate dentinal tubules. Both Capasio and MTA promote apatite deposition when exposed to STF.
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