Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents.
Identity and mechanisms of alkane-oxidizing metalloenzymes from deep-sea hydrothermal vents.
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DOI：
10.3389/fmicb.2013.00109
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发表时间：
2013
影响因子：
5.2
通讯作者：
Austin RN
中科院分区：
文献类型：
--
作者：
Bertrand EM;Keddis R;Groves JT;Vetriani C;Austin RN
六个好氧链烷营养菌（生物体，可以代谢烷烃作为其唯一的碳源）分离自深海热液喷口的特点是使用自由基时钟底物降冰片烷，以确定金属酶和反应机制用于氧化烷烃。所研究的微生物为食烷菌属菌株EPR 7和MAR 14、海蛞蝓属菌株EPR 21、类诺卡氏菌属菌株EPR 26 w、EPR 28 w和破烃小杆菌菌株EPR 92。每种生物都能以正构烷烃为唯一碳源生长，因此必须表达编码烷烃氧化酶的基因。自由基时钟诊断底物降冰片烷的氧化结果表明，六种生物体中的五种（EPR 7，MAR 14，EPR 21，EPR 26 w和EPR 28 w）使用与AlkB功能相似的烷烃羟化酶催化中链烷烃的氧化，而第六种生物体（EPR 92）使用烷烃氧化细胞色素P450（P450）样蛋白催化氧化。DNA测序表明，EPR7和EPR21具有编码AlkB蛋白的基因，而EPR92的测序结果证实了编码CYP样烷烃羟化酶的基因的存在，与降冰片烷实验的结果一致。
Six aerobic alkanotrophs (organism that can metabolize alkanes as their sole carbon source) isolated from deep-sea hydrothermal vents were characterized using the radical clock substrate norcarane to determine the metalloenzyme and reaction mechanism used to oxidize alkanes. The organisms studied were Alcanivorax sp. strains EPR7 and MAR14, Marinobacter sp. strain EPR21, Nocardioides sp. strains EPR26w, EPR28w, and Parvibaculum hydrocarbonoclasticum strain EPR92. Each organism was able to grow on n-alkanes as the sole carbon source and therefore must express genes encoding an alkane-oxidizing enzyme. Results from the oxidation of the radical-clock diagnostic substrate norcarane demonstrated that five of the six organisms (EPR7, MAR14, EPR21, EPR26w, and EPR28w) used an alkane hydroxylase functionally similar to AlkB to catalyze the oxidation of medium-chain alkanes, while the sixth organism (EPR92) used an alkane-oxidizing cytochrome P450 (CYP)-like protein to catalyze the oxidation. DNA sequencing indicated that EPR7 and EPR21 possess genes encoding AlkB proteins, while sequencing results from EPR92 confirmed the presence of a gene encoding CYP-like alkane hydroxylase, consistent with the results from the norcarane experiments.
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影响因子：
16.6
作者：
Austin, Rachel N.;Luddy, Kate;Groves, John T.
通讯作者：
Groves, John T.
DOI：
10.1016/j.ibiod.2011.12.008
发表时间：
2012-04-01
影响因子：
4.8
作者：
Al-Awadhi, H.;Dashti, N.;Radwan, S.
通讯作者：
Radwan, S.
影响因子：
3.9
作者：
Bertrand, E;Sakai, R;Austin, RN
通讯作者：
Austin, RN
影响因子：
15
作者：
Brazeau, BJ;Austin, RN;Lipscomb, JD
通讯作者：
Lipscomb, JD
影响因子：
3
作者：
BRAULT, M;SIMONEIT, BRT;SALIOT, A
通讯作者：
SALIOT, A