Identification of novel arthropod vector G protein-coupled receptors.
Identification of novel arthropod vector G protein-coupled receptors.
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DOI：
10.1186/1756-3305-6-150
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发表时间：
2013-05-24
影响因子：
3.2
通讯作者：
Izaguirre JA
中科院分区：
文献类型：
--
作者：
Nowling RJ;Abrudan JL;Shoue DA;Abdul-Wahid B;Wadsworth M;Stayback G;Collins FH;McDowell MA;Izaguirre JA
病媒传播疾病，如疟疾、登革热和斑疹伤寒的控制往往是通过使用杀虫剂来实现的。不幸的是，杀虫剂抗药性在不同病媒物种中越来越普遍。目前还没有化学替代品来替代这些杀虫剂，因为新的对人类安全的分子类别尚未进入病媒控制市场。新靶点的确定为合理设计控制病媒种群的新化学品提供了机会。一个目标家族，G蛋白偶联受体（GPCR），在杀虫剂开发方面仍然相对不足。开发了一种用于载体GPCR的新型分类器EnligHTN *。使用一组来自埃及伊蚊、冈比亚按蚊、意大利蜜蜂、黑腹果蝇、智人和人类马足的所有已知GPCR，对Entrance * 进行验证并与现有分类器进行比较。来自Ae的未鉴定序列的预测。aegypti、埃及按蚊An. gambiae和Pe. humanus是有效的。对已知和新发现的Ae.埃及GPCR基因。我们提出了一个新的分析基因组中的GPCR的埃及伊蚊，登革热的载体，一个。gambiae，引起疟疾的恶性疟原虫的主要载体，和Pe. humanus，流行性斑疹伤寒的载体，使用一种新的GPCR分类器，Enhancer *，设计用于昆虫载体物种。我们确定了另外30个假定的GPCR，其中19个我们验证。新发现的AE的表达。通过定量RT-PCR证实埃及GPCR基因。开发了一种用于昆虫载体的新型GPCR分类器Envelope *，并验证了GPCR预测。Enhancement * 和验证流水线应用于三种昆虫载体的基因组（Ae. aegypti、埃及按蚊An. gambiae和Pe. humanus），导致鉴定了52个先前未鉴定的GPCR，其中11个是预测的GPCR，19个是预测和确认的GPCR。
The control of vector-borne diseases, such as malaria, dengue fever, and typhus fever is often achieved with the use of insecticides. Unfortunately, insecticide resistance is becoming common among different vector species. There are currently no chemical alternatives to these insecticides because new human-safe classes of molecules have yet to be brought to the vector-control market. The identification of novel targets offer opportunities for rational design of new chemistries to control vector populations. One target family, G protein-coupled receptors (GPCRs), has remained relatively under explored in terms of insecticide development. A novel classifier, Ensemble*, for vector GPCRs was developed. Ensemble* was validated and compared to existing classifiers using a set of all known GPCRs from Aedes aegypti, Anopheles gambiae, Apis Mellifera, Drosophila melanogaster, Homo sapiens, and Pediculus humanus. Predictions for unidentified sequences from Ae. aegypti, An. gambiae, and Pe. humanus were validated. Quantitative RT-PCR expression analysis was performed on previously-known and newly discovered Ae. aegypti GPCR genes. We present a new analysis of GPCRs in the genomes of Ae, aegypti, a vector of dengue fever, An. gambiae, a primary vector of Plasmodium falciparum that causes malaria, and Pe. humanus, a vector of epidemic typhus fever, using a novel GPCR classifier, Ensemble*, designed for insect vector species. We identified 30 additional putative GPCRs, 19 of which we validated. Expression of the newly discovered Ae. aegypti GPCR genes was confirmed via quantitative RT-PCR. A novel GPCR classifier for insect vectors, Ensemble*, was developed and GPCR predictions were validated. Ensemble* and the validation pipeline were applied to the genomes of three insect vectors (Ae. aegypti, An. gambiae, and Pe. humanus), resulting in the identification of 52 GPCRs not previously identified, of which 11 are predicted GPCRs, and 19 are predicted and confirmed GPCRs.
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影响因子：
4.1
作者：
Gloriam, DEI;Bjarnadóttir, TK;Fredriksson, R
通讯作者：
Fredriksson, R
影响因子：
14.9
作者：
Horn, F;Bettler, E;Vriend, G
通讯作者：
Vriend, G
影响因子：
3.6
作者：
Fredriksson, R;Schiöth, HB
通讯作者：
Schiöth, HB
影响因子：
14.9
作者：
Hubbard, T;Barker, D;Clamp, M
通讯作者：
Clamp, M
DOI：
10.1016/j.bbrc.2005.10.123
发表时间：
2005-12-23
影响因子：
3.1
作者：
Inoue, Y;Yamazaki, Y;Shimizu, T
通讯作者：
Shimizu, T