Gene expression profile of isolated rat adipocytes treated with anthocyanins.
Gene expression profile of isolated rat adipocytes treated with anthocyanins.
复制标题
用花青素处理的分离的大鼠脂肪细胞的基因表达谱。
DOI：
10.1016/j.bbalip.2004.12.014
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
T. Osawa
中科院分区：
文献类型：
--
作者：
T. Tsuda;Y. Ueno;H. Kojo;T. Yoshikawa;T. Osawa
脂肪细胞功能障碍与肥胖和胰岛素抵抗的发展密切相关。调节脂肪细胞因子的分泌或脂肪细胞特异性基因的表达是预防肥胖和改善胰岛素敏感性的重要靶点之一。近年来，我们发现花青素作为一种广泛存在于植物界的色素，具有抗小鼠肥胖、促进脂肪细胞中脂肪细胞因子分泌和脂肪细胞基因表达的作用。在这项研究中，我们已经显示了第一次在离体大鼠脂肪细胞处理花青素（花青素3-葡萄糖苷; C3 G或花青素; Cy）的基因表达谱。用100 μM C3 G、Cy或溶剂处理大鼠脂肪细胞24 h。从脂肪细胞中提取总RNA并进行基因芯片分析。分别用C3 G或Cy处理脂肪细胞，总共有633或427个基因上调（>1.5倍）。上调的基因包括脂代谢和信号转导相关基因，但C3 G和Cy-treated组之间改变的基因部分不同。基于基因表达谱，我们证明了激素敏感性脂肪酶的上调和脂肪分解活性的增强与C3 G或Cy处理的脂肪细胞。这些数据提供了花青素处理的脂肪细胞中基因表达谱的概述，并确定了新的响应基因，这些基因在与肥胖和糖尿病相关的脂肪细胞中具有潜在的重要功能，值得进一步研究。
Adipocyte dysfunction is strongly associated with the development of obesity and insulin resistance. It is accepted that the regulation of adipocytokine secretion or the adipocyte specific gene expression is one of the most important targets for the prevention of obesity and amelioration of insulin sensitivity. Recently, we demonstrated that anthocyanins, which are pigments widespread in the plant kingdom, have the potency of anti-obesity in mice and the enhancement adipocytokine secretion and adipocyte gene expression in adipocytes. In this study, we have shown for the first time the gene expression profile in isolated rat adipocytes treated with anthocyanins (cyanidin 3-glucoside; C3G or cyanidin; Cy). The rat adipocytes were treated with 100 μM C3G, Cy or vehicle for 24 h. The total RNA from the adipocytes was isolated and carried out GeneChip microarray analysis. A total of 633 or 427 genes was up-regulated (>1.5-fold) by the treatment of adipocytes with C3G or Cy, respectively. The up-regulated genes include lipid metabolism and signal transduction-related genes, however, the altered genes were partly different between the C3G- and Cy-treated groups. Based on the gene expression profile, we demonstrated the up-regulation of hormone sensitive lipase and enhancement of the lipolytic activity by the treatment of adipocytes with C3G or Cy. These data have provided an overview of the gene expression profiles in adipocytes treated with anthocyanins and identified new responsive genes with potentially important functions in adipocytes related with obesity and diabetes that merit further investigation.
影响因子：
6.5
作者：
B. Slavin;J. Ong;P. Kern
通讯作者：
B. Slavin;J. Ong;P. Kern