High olive oil diets enhance cervical tumour growth in mice: transcriptome analysis for potential candidate genes and pathways
High olive oil diets enhance cervical tumour growth in mice: transcriptome analysis for potential candidate genes and pathways
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高橄榄油饮食促进小鼠宫颈肿瘤生长：潜在候选基因和途径的转录组分析
DOI：
10.1186/s12944-019-1023-6
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发表时间：
2019-03
影响因子：
4.5
通讯作者：
Chen Yaxi
中科院分区：
文献类型：
--
作者：
Zhang Xiaoyu;Yang Ping;Luo Xuan;Su Chunxiao;Chen Yao;Zhao Lei;Wei Li;Zeng Han;Varghese Zac;Moorhead John F;Ruan Xiong Z;Chen Yaxi
背景大量流行病学研究发现肥胖与癌症之间存在密切关联。膳食脂肪是肥胖的一个根本原因，也是癌症的危险因素。迄今为止，膳食橄榄油对癌症发展的影响仍无定论，对其潜在机制知之甚少。方法采用裸鼠异种移植模型来研究高橄榄油饮食喂养对宫颈癌（CC）发生和进展的影响。分别采用EdU掺入法、伤口愈合法和Transwell实验法观察细胞增殖、迁移和侵袭。 RNA测序技术和综合生物信息学分析被用来阐明膳食脂肪调节的分子过程。通过基因本体论 (GO)、京都基因和基因组富集 (KEGG) 鉴定差异表达基因 (DEG) 并进行功能分析。然后，使用STRING数据库和Cytoscape软件进行蛋白质-蛋白质相互作用（PPI）网络和亚PPI网络分析。结果高橄榄油饮食加剧了CC实验异种移植模型中的肿瘤发生。油酸是橄榄油的主要成分，在体外促进细胞生长和迁移。然后对异种移植肿瘤组织进行转录组测序分析，以阐明膳食脂肪调节分子事件的调节。膳食橄榄油诱导了 648 个 DEG，其中 155 个上调 DEG 和 493 个下调 DEG。 GO和通路富集分析显示，一些DEG（包括EGR1和FOXN2）参与转录调控，其他DEG（包括TGFB2和COL4A3）参与细胞增殖。通过实时定量 PCR 分析，从 PPI 网络中选出了 15 个相关性最强的 DEG，包括 JUN、TIMP3、OAS1、OASL 和 EGR1 在内的枢纽基因。结论我们的研究表明，高橄榄油饮食会加剧体内和体外 CC 的进展。我们提供线索来建立膳食脂肪与癌症发生之间的潜在联系，并确定需要进一步研究的领域。
BackgroundNumerous epidemiologic studies have found a close association between obesity and cancer. Dietary fat is a fundamental contributor to obesity and is a risk factor for cancer. Thus far, the impact of dietary olive oil on cancer development remains inconclusive, and little is known about its underlying mechanisms.MethodsNude mouse xenograft models were used to examine the effects of high olive oil diet feeding on cervical cancer (CC) development and progression. Cell proliferation, migration and invasion were observed by the methods of EdU incorporation, Wound healing and Transwell assay, separately. RNA-sequencing technology and comprehensive bioinformatics analyses were used to elucidate the molecular processes regulated by dietary fat. Differentially expressed genes (DEGs) were identified and were functionally analyzed by Gene Ontology (GO), Kyoto Enrichment of Genes and Genomes (KEGG). Then, protein–protein interaction (PPI) network and sub-PPI network analyses were conducted using the STRING database and Cytoscape software.ResultsA high olive oil diet aggravated tumourigenesis in an experimental xenograft model of CC. Oleic acid, the main ingredient of olive oil, promoted cell growth and migration in vitro. Transcriptome sequencing analysis of xenograft tumour tissues was then performed to elucidate the regulation of molecular events regulated by dietary fat. Dietary olive oil induced 648 DEGs, comprising 155 up-regulated DEGs and 493 down-regulated DEGs. GO and pathway enrichment analysis revealed that some of the DEGs including EGR1 and FOXN2 were involved in the transcription regulation and others, including TGFB2 and COL4A3 in cell proliferation. The 15 most strongly associated DEGs were selected from the PPI network and hub genes including JUN, TIMP3, OAS1, OASL and EGR1 were confirmed by real-time quantitative PCR analysis.ConclusionsOur study suggests that a high olive oil diet aggravates CC progression in vivo and in vitro. We provide clues to build a potential link between dietary fat and cancerogenesis and identify areas requiring further investigation.
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影响因子：
2
作者：
R. Dmochowski
通讯作者：
R. Dmochowski
DOI：
10.1016/j.respe.2020.09.004
发表时间：
2020-10-10
期刊：
Revue D'Epidemiologie et De Sante Publique
影响因子：
--
作者：
La rédaction
通讯作者：
La rédaction
影响因子：
9.3
作者：
通讯作者：
--
影响因子：
5.9
作者：
Craighead FL
通讯作者：
Craighead FL