Protective antitumor activity through dendritic cell immunization is mediated by NK cell as well as CTL activation
Protective antitumor activity through dendritic cell immunization is mediated by NK cell as well as CTL activation
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通过树突状细胞免疫产生的保护性抗肿瘤活性由 NK 细胞以及 CTL 激活介导
DOI：
10.1007/bf02979055
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发表时间：
1999
影响因子：
6.7
通讯作者：
Jong
中科院分区：
文献类型：
--
作者：
Kwang Dong Kim;Jin Koo Kim;Se;I. Choe;Tae;Y. Choe;Jong
树突状细胞(DC)是一种功能强大的专职抗原提呈细胞(APC)，能够诱导T细胞对抗原的初级反应。尽管肿瘤细胞表达靶抗原，但由于T细胞最佳激活所需的共刺激信号的缺陷，它们不能刺激肿瘤特异性免疫反应。在这项工作中，我们描述了一种新的方法，使用肿瘤-DC共培养来提高肿瘤细胞的抗原提呈能力，这种方法不需要肿瘤相关抗原源。免疫与骨髓来源的DC共培养的弱免疫原性和进展性肿瘤产生了有效的肿瘤疫苗。共培养树突状细胞免疫能够诱导对肿瘤攻击的完全保护性免疫，并能有效地诱导肿瘤特异性CTL(细胞毒性T淋巴细胞)活性。此外，在肿瘤被排斥的小鼠中观察到高NK细胞活性。此外，肿瘤致敏的树突状细胞免疫可延缓荷瘤小鼠的肿瘤生长，但不能消除肿瘤。我们的结果表明，DC与肿瘤共培养后，由于NK细胞的激活和肿瘤特异性T细胞的产生，产生了抗肿瘤免疫。当有关肿瘤抗原的信息有限时，这种方法对于使用DC设计肿瘤疫苗是有用的。
Dendritic cells (DCs) are potent professional antigen-presenting cells (APC) capable of inducing the primary T cell response to antigen. Although tumor cells express target antigens, they are incapable of stimulating a tumor-specific immune response due to a defect in the costimulatory signal that is required for optimal activation of T cells. In this work, we describe a new approach using tumor-DC coculture to improve the antigen presenting capacity of tumor cells, which does not require a source of tumor-associated antigen. Immunization of a weakly immunogenic and progressive tumor cocultured with bone marrow-derived DCs generated an effective tumor vaccine. Immunization with the cocultured DCs was able to induce complete protective immunity against tumor challenges and was effective for the induction of tumor-specific CTL (cytotoxic T lymphocyte) activity. Furthermore, high NK cell activity was observed in mice in which tumors were rejected. In addition, immunization with tumor-pulsed DCs induced delayed tumor growth, but not tumor eradication in tumor-bearing mice. Our results demonstrate that coculture of DCs with tumors generated antitumor immunity due to the NK cell activation as well as tumor-specific T cell. This approach would be useful for designing tumor vaccines using DCs when the information about tumor antigens is limited.
DOI：
10.1016/0167-5699(96)80616-5
发表时间：
1996-04-01
期刊：
IMMUNOLOGY TODAY
影响因子：
--
作者：
Kos, FJ;Engleman, EG
通讯作者：
Engleman, EG