Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4 pretreatments in Miscanthus.
Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4 pretreatments in Miscanthus.
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半纤维素对木质纤维素的结晶度负面影响，以在MISCANTHUS中NaOH和H2SO4预处理下进行高生物质消化率。
DOI：
10.1186/1754-6834-5-58
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发表时间：
2012-08-11
影响因子：
6.3
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Xu N;Zhang W;Ren S;Liu F;Zhao C;Liao H;Xu Z;Huang J;Li Q;Tu Y;Yu B;Wang Y;Jiang J;Qin J;Peng L
木质纤维素是地球上最丰富的生物质。然而，生物质可再生性已成为影响生物燃料生产的主要因素。虽然纤维素结晶度显着影响生物质糖化，鲜为人知的是三个主要的壁聚合物对纤维素结晶的影响。在这项研究中，我们选择了六对典型的芒样品，呈现不同的细胞壁组成，然后比较其纤维素结晶度和生物质消化率后，各种化学预处理。具有高半纤维素水平的芒草样品被确定为具有相对低的纤维素结晶度指数（CrI），并且在用三种浓度的NaOH和H2SO4预处理后以类似的速率增强生物质消化率。相比之下，具有高纤维素或木质素水平的芒草样品显示出增加的CrI和低生物质糖化，特别是在H2SO4预处理后。相关性分析表明，纤维素CrI的负面影响生物质消化。半纤维素水平增加25%或纤维素和木质素含量减少31%和37%，也被发现导致增加的己糖产量由1.3倍至2.2倍释放从酶水解后NaOH或H2SO4预处理。结果表明，半纤维素是影响生物质消化率的主要因素，纤维素和木质素对生物质消化率有协同负效应。使用六对芒样品具有不同的细胞壁组成，半纤维素被揭示为主导因素，积极确定生物质消化率预处理后，用NaOH或H2SO4的负面影响纤维素结晶度。这些结果为生物能源作物的遗传改良提供了潜在的途径。
Lignocellulose is the most abundant biomass on earth. However, biomass recalcitrance has become a major factor affecting biofuel production. Although cellulose crystallinity significantly influences biomass saccharification, little is known about the impact of three major wall polymers on cellulose crystallization. In this study, we selected six typical pairs of Miscanthus samples that presented different cell wall compositions, and then compared their cellulose crystallinity and biomass digestibility after various chemical pretreatments. A Miscanthus sample with a high hemicelluloses level was determined to have a relatively low cellulose crystallinity index (CrI) and enhanced biomass digestibility at similar rates after pretreatments of NaOH and H2SO4 with three concentrations. By contrast, a Miscanthus sample with a high cellulose or lignin level showed increased CrI and low biomass saccharification, particularly after H2SO4 pretreatment. Correlation analysis revealed that the cellulose CrI negatively affected biomass digestion. Increased hemicelluloses level by 25% or decreased cellulose and lignin contents by 31% and 37% were also found to result in increased hexose yields by 1.3-times to 2.2-times released from enzymatic hydrolysis after NaOH or H2SO4 pretreatments. The findings indicated that hemicelluloses were the dominant and positive factor, whereas cellulose and lignin had synergistic and negative effects on biomass digestibility. Using six pairs of Miscanthus samples with different cell wall compositions, hemicelluloses were revealed to be the dominant factor that positively determined biomass digestibility after pretreatments with NaOH or H2SO4 by negatively affecting cellulose crystallinity. The results suggested potential approaches to the genetic modifications of bioenergy crops.
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影响因子：
4.1
作者：
JUNG, HJG;BUXTON, DR
通讯作者：
BUXTON, DR
影响因子：
6.3
作者：
Park S;Baker JO;Himmel ME;Parilla PA;Johnson DK
通讯作者：
Johnson DK
影响因子：
11.4
作者：
Huang, Jiangfeng;Xia, Tao;Peng, Liangcai
通讯作者：
Peng, Liangcai
影响因子：
7.7
作者：
Ralph, John;Lundquist, Knut;Boerjan, Wout
通讯作者：
Boerjan, Wout
纤维素的获取限制了酶水解的效率：无定形发生的作用。
影响因子：
6.3
作者：
Arantes V;Saddler JN
通讯作者：
Saddler JN