Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis.
Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis.
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纤维素的获取限制了酶水解的效率：无定形发生的作用。
DOI：
10.1186/1754-6834-3-4
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发表时间：
2010-02-23
影响因子：
6.3
通讯作者：
Saddler JN
中科院分区：
文献类型：
--
作者：
Arantes V;Saddler JN
在低纤维素酶（蛋白质）负载下对纤维素进行有效的酶促糖化仍然是木质纤维素生物转化为乙醇的工艺商业化的挑战。目前，需要有效的预处理和高酶负载量来克服限制生物质底物的纤维素部分快速和完全水解的几种底物和酶因素。纤维素酶面临的主要障碍之一是它们对大部分纤维素的获取有限，而这些纤维素埋藏在高度有序且紧密堆积的纤维素微纤维结构中。有人建议，这些不可接近的区域被非水解蛋白质破坏或松开，而不是从外部连续“剃须”或“刨平”纤维素原纤维，从而增加纤维素表面积并使纤维素酶复合物更容易接近。纤维素酶促糖化的初始阶段被称为无定形发生。在这篇综述中，我们描述了已提出的各种无定形诱导剂，以及它们在增强纤维素酶水解中的可能作用。
The efficient enzymatic saccharification of cellulose at low cellulase (protein) loadings continues to be a challenge for commercialization of a process for bioconversion of lignocellulose to ethanol. Currently, effective pretreatment followed by high enzyme loading is needed to overcome several substrate and enzyme factors that limit rapid and complete hydrolysis of the cellulosic fraction of biomass substrates. One of the major barriers faced by cellulase enzymes is their limited access to much of the cellulose that is buried within the highly ordered and tightly packed fibrillar architecture of the cellulose microfibrils. Rather than a sequential 'shaving' or 'planing' of the cellulose fibrils from the outside, it has been suggested that these inaccessible regions are disrupted or loosened by non-hydrolytic proteins, thereby increasing the cellulose surface area and making it more accessible to the cellulase enzyme complex. This initial stage in enzymatic saccharification of cellulose has been termed amorphogenesis. In this review, we describe the various amorphogenesis-inducing agents that have been suggested, and their possible role in enhancing the enzymatic hydrolysis of cellulose.
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影响因子：
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