Revealing pancrustacean relationships: Phylogenetic analysis of ribosomal protein genes places Collembola (springtails) in a monophyletic Hexapoda and reinforces the discrepancy between mitochondrial and nuclear DNA markers
Revealing pancrustacean relationships: Phylogenetic analysis of ribosomal protein genes places Collembola (springtails) in a monophyletic Hexapoda and reinforces the discrepancy between mitochondrial and nuclear DNA markers
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揭示泛甲壳类动物的关系：核糖体蛋白基因的系统发育分析将跳虫（跳虫）置于单系六足动物中，并强化了线粒体和核 DNA 标记之间的差异
DOI：
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发表时间：
2008
影响因子：
3.4
通讯作者：
N. Straalen
中科院分区：
文献类型：
--
作者：
M. Timmermans;D. Roelofs;J. Mariën;N. Straalen
背景近年来，基于DNA序列的研究提出了几种新的节肢动物系统发育关系假说。其中一个受到挑战的假设是六足动物的单系性。这一讨论起源于基于线粒体DNA数据集的分析，由于弹尾目的不寻常定位，表明六足动物的身体计划至少进化了两次。在这里，我们重新评估的位置弹尾虫核糖体蛋白基因sequences.ResultsIn共获得48核糖体蛋白的弹尾虫Folsomia念珠菌。这48个序列与其他35个蜕皮动物的序列数据进行了比对。每个核糖体蛋白基因可用于25%至86%的分类群。然而，总的序列信息是不均匀分布的类群，范围在4%和100%之间。构建串联数据集（长度为5034个推断的氨基酸），其中约66%的位置被填充。系统发育树重建，使用最大似然，最大简约，贝叶斯方法，导致在拓扑结构，支持单系的Hexapoda.ConclusionAlthough核糖体蛋白一般可能不会独立发展，他们再次出现非常有价值的系统发育重建。我们的分析清楚地表明，六足类是单系的。在分析泛甲壳动物关系时，这支持了核和线粒体数据集之间的不一致性。在应用线粒体标记物研究深源遗传学时需要谨慎。
BackgroundIn recent years, several new hypotheses on phylogenetic relations among arthropods have been proposed on the basis of DNA sequences. One of the challenged hypotheses is the monophyly of hexapods. This discussion originated from analyses based on mitochondrial DNA datasets that, due to an unusual positioning of Collembola, suggested that the hexapod body plan evolved at least twice. Here, we re-evaluate the position of Collembola using ribosomal protein gene sequences.ResultsIn total 48 ribosomal proteins were obtained for the collembolan Folsomia candida. These 48 sequences were aligned with sequence data on 35 other ecdysozoans. Each ribosomal protein gene was available for 25% to 86% of the taxa. However, the total sequence information was unequally distributed over the taxa and ranged between 4% and 100%. A concatenated dataset was constructed (5034 inferred amino acids in length), of which ~66% of the positions were filled. Phylogenetic tree reconstructions, using Maximum Likelihood, Maximum Parsimony, and Bayesian methods, resulted in a topology that supports monophyly of Hexapoda.ConclusionAlthough ribosomal proteins in general may not evolve independently, they once more appear highly valuable for phylogenetic reconstruction. Our analyses clearly suggest that Hexapoda is monophyletic. This underpins the inconsistency between nuclear and mitochondrial datasets when analyzing pancrustacean relationships. Caution is needed when applying mitochondrial markers in deep phylogeny.
影响因子：
7
作者：
Stajich, JE;Block, D;Birney, E
通讯作者：
Birney, E
DOI：
10.1006/bbrc.1995.2518
发表时间：
1995-10-13
影响因子：
3.1
作者：
CHAN, YL;SUZUKI, K;WOOL, IG
通讯作者：
WOOL, IG